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Titolo:
IDENTIFICATION OF THE GENE ENCODING THE MAJOR CAPSID PROTEIN OF INSECT IRIDESCENT VIRUS TYPE-6 BY POLYMERASE CHAIN-REACTION
Autore:
STOHWASSER R; RAAB K; SCHNITZLER P; JANSSEN W; DARAI G;
Indirizzi:
UNIV HEIDELBERG,INST MED VIROL,NEUENHEIMER FELD 324 W-6900 HEIDELBERGGERMANY UNIV HEIDELBERG,INST MED VIROL,NEUENHEIMER FELD 324 W-6900 HEIDELBERGGERMANY
Titolo Testata:
Journal of General Virology
, volume: 74, anno: 1993,
parte:, 5
pagine: 873 - 879
SICI:
0022-1317(1993)74:<873:IOTGET>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAIN HALLNAS B1; MOLECULAR-CLONING; CIRCULAR PERMUTATION; DNA; RNA; SEGMENT; GENOME; POLYPEPTIDES; SEQUENCES; PLASMIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
R. Stohwasser et al., "IDENTIFICATION OF THE GENE ENCODING THE MAJOR CAPSID PROTEIN OF INSECT IRIDESCENT VIRUS TYPE-6 BY POLYMERASE CHAIN-REACTION", Journal of General Virology, 74, 1993, pp. 873-879

Abstract

The gene encoding the major capsid protein of Chilo iridescent virus (CIV) has been identified by PCR using oligonucleotide primers corresponding to different regions of the major capsid proteins of Tipula iridescent virus (TIV) and iridescent virus 22 (IV22). A DNA fragment of 0.5 kbp was amplified using two oligonucleotide primers corresponding to the amino acid positions 146 to 153 and 304 to 313 of the major capsid protein of TIV, respectively. The radioactively labelled DNA fragment derived from PCR was hybridized to a CIV gene library. This analysis revealed that only the EcoRI CIV DNA fragment X [2.85 kbp; 0.589 to0.603 viral map units (m.u.)] hybridized to the amplified DNA fragment. An RNA transcript of about 1.5 kb was identified when the PCR product was used as a hybridization probe. The same RNA transcript was detected when the EcoRI fragments X and Q (5.9 kbp; 0.603 to 0.631 viral m.u.) were used as probes. This indicates that the expected gene is located within map coordinates 0-589 to 0-631 and harbours part of the DNA sequences of fragments Q and X. The analysis of the DNA sequences ofthis particular region of the CIV genome revealed the presence of oneopen reding frame of 1401 bp. The DNA sequences of this region encodea protein of 467 amino acid residues with an M(r) of 51.4K. A high degree (64.7 %) of amino acid sequence identity was detected between themajor capsid protein of TIV and/or IV22 and the amino acid composition of the identified CIV protein.

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Documento generato il 03/12/20 alle ore 15:55:34