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Titolo:
INFLUENCE OF BUFFER COMPOSITION ON PROTEIN ADSORPTION ON AGAROSE (SEPHAROSE-6B)
Autore:
FORNSTEDT N; FORNSTEDT T; PORATH J;
Indirizzi:
UNIV UPPSALA,CTR BIOCHEM SEPARAT,BMC,POB 577 S-75123 UPPSALA SWEDEN UNIV UPPSALA,DEPT ANALYT PHARMACEUT CHEM,BMC S-75123 UPPSALA SWEDEN UNIV ARIZONA,DIV BIOTECHNOL TUCSON AZ 85721
Titolo Testata:
Chromatographia
fascicolo: 7-8, volume: 35, anno: 1993,
pagine: 387 - 389
SICI:
0009-5893(1993)35:7-8<387:IOBCOP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Keywords:
COLUMN LIQUID CHROMATOGRAPHY; BUFFER COMPOSITION EFFECT; AGAROSE; ALBUMIN; IONIC ADSORPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
3
Recensione:
Indirizzi per estratti:
Citazione:
N. Fornstedt et al., "INFLUENCE OF BUFFER COMPOSITION ON PROTEIN ADSORPTION ON AGAROSE (SEPHAROSE-6B)", Chromatographia, 35(7-8), 1993, pp. 387-389

Abstract

The adsorption of albumin on a commercial, high quality agarose (Sepharose(R) 6B) was studied in different buffers of low pH (4.00) and lowmolarity (9-11 mM). Eluents containing 0.025 % albumin were introduced onto small gel columns until saturation and the adsorption capacity of the gel in different buffers calculated. Adsorption was high in solutions of monobasic acids. Although cation-exchange interactions between the protein and the gel matrix were found to be influenced essentially by the sodium ion concentration or ionic strength of the buffer, adsorption in citrate buffers is weak compared to that in formate, acetate or propionate buffers of about the same ionic strength (I = 0.01).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 18:18:42