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Titolo:
WHOLE-CELL AND SINGLE-CHANNEL ANALYSIS OF THE KINETICS OF GLYCINE-SENSITIVE N-METHYL-D-ASPARTATE RECEPTOR DESENSITIZATION
Autore:
PARSONS CG; ZONG XG; LUX HD;
Indirizzi:
MERZ & CO GMBH & CO,ECKENHEIMER LANDSTR 100-104 W-6000 FRANKFURT 1 GERMANY MAX PLANCK INST PSYCHIAT,DEPT NEUROPHYSIOL W-8033 PLANEGG GERMANY
Titolo Testata:
British Journal of Pharmacology
fascicolo: 1, volume: 109, anno: 1993,
pagine: 213 - 221
SICI:
0007-1188(1993)109:1<213:WASAOT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED HIPPOCAMPAL-NEURONS; TRANSIENT GLOBAL-ISCHEMIA; XENOPUS-OOCYTES; RAT-BRAIN; COMPETITIVE ANTAGONISTS; MK-801 BINDING; ACID RECEPTORS; NMDA RECEPTORS; GLUTAMATE; RESPONSES;
Keywords:
DESENSITIZATION; N-METHYL-D-ASPARTATE (NMDA); GLYCINE; PATCH CLAMP; ELECTROPHYSIOLOGY; SUPERIOR COLLICULUS CULTURED NEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
C.G. Parsons et al., "WHOLE-CELL AND SINGLE-CHANNEL ANALYSIS OF THE KINETICS OF GLYCINE-SENSITIVE N-METHYL-D-ASPARTATE RECEPTOR DESENSITIZATION", British Journal of Pharmacology, 109(1), 1993, pp. 213-221

Abstract

1 The kinetics of glycine-sensitive, N-methyl-D-aspartate (NMDA) receptor desensitization were investigated in cultured neurones with the patch clamp technique. 2 The degree of fast NMDA-receptor desensitization was inversely related to glycine concentration. Thus, increasing concentrations of glycine from 30 nm to 2.5 mum potentiated desensitizedNMDA responses (873% +/- 101%) to a greater degree than peak responses (260% +/-27%). 3 The desensitization was due to a decrease in the affinity of glycine for the strychnine-insensitive, glycine modulatory site (glycine(B) site) following activation of the NMDA-receptor complex. Thus, the A50 for glycine in potentiating peak responses (77 nm, 95% confidence limited 58-104 nm) was five fold lower than that for plateau responses (399 nm, 340-468 nm). 4 The rate of desensitization was related to glycine concentration such that a reciprocal plot of desensitization rate (1/tau S-1) against glycine concentration had a slope of 9.510(6) M-1 S-1. 5 Recovery from desensitization following step increases in glycine or L-alanine concentration in the continuous presence of NMDA (200 mum) reflected the association kinetics of the glycine, agonist used. 6 The rate and degree of NMDA receptor desensitizationwas independent of holding potential. 7 NMDA receptor desensitizationwas also evident at the single channel level. 8 The glycine(B) antagonist 7-chlorokynurenic acid (7-Chl-Kyn 3 and 10 mum) concentration-dependently induced an identical form of desensitization in the presence of 1 mum glycine. 9 In contrast, the competitive NMDA antagonist (+/-)-amino-phosphonovaleric acid (APV 30 to 300 mum) concentration-dependently antagonized and slowed the onset kinetics of NMDA responses.

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Documento generato il 11/07/20 alle ore 11:24:57