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Titolo:
DETECTION OF INSITU HYBRIDIZATION TO HUMAN-CHROMOSOMES WITH THE ATOMIC FORCE MICROSCOPE
Autore:
PUTMAN CAJ; DEGROOTH BG; WIEGANT J; RAAP AK; VANDERWERF KO; VANHULST NF; GREVE J;
Indirizzi:
TWENTE UNIV TECHNOL,DEPT APPL PHYS 7500 AE ENSCHEDE NETHERLANDS LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY 2333 AL LEIDEN NETHERLANDS
Titolo Testata:
Cytometry
fascicolo: 4, volume: 14, anno: 1993,
pagine: 356 - 361
SICI:
0196-4763(1993)14:4<356:DOIHTH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA;
Keywords:
HIGH-RESOLUTION MICROSCOPY; GENE MAPPING; DNA PROBES PUC1.77 AND P1-79;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
C.A.J. Putman et al., "DETECTION OF INSITU HYBRIDIZATION TO HUMAN-CHROMOSOMES WITH THE ATOMIC FORCE MICROSCOPE", Cytometry, 14(4), 1993, pp. 356-361

Abstract

Atomic force microscopy (AFM) permits one to generate a topographic representation of the sample under investigation with high spatial resolution. We assumed that cytochemical staining techniques, which yield reaction products which can be discriminated from the surrounding material on basis of their topographic properties, would be applicable in AFM. Here we show the validity of this assumption by employing an in situ hybridization technique in which the final label was the precipitated product of a peroxidase/diaminebenzidine reaction. After hybridization of the DNA probe pUC1.77 that recognizes the heterochromatic region of human chromosome 1 (1q12), the AFM clearly detects the sites of in situ hybridization. In situ hybridization with DNA probe p1-79 results in clear marking of the telomere region 1p36. The diameter of the probe p1-79 linked reaction product was 75-100 nm, indicating that resolution of 200 nm can readily be reached with this AFM approach of DNAmapping. This precision is directly linked with the amount of precipitated material.

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Documento generato il 01/12/20 alle ore 00:45:22