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Titolo:
DELIVERY OF WHOLE LIVER EQUIVALENT HEPATOCYTE MASS USING POLYMER DEVICES AND HEPATOTROPHIC STIMULATION
Autore:
UYAMA S; KAUFMANN PM; TAKEDA T; VACANTI JP;
Indirizzi:
CHILDRENS HOSP MED CTR,DEPT SURG BOSTON MA 02115 HARVARD UNIV,SCH MED BOSTON MA 02115 KYOTO UNIV,DEPT SURG 2 KYOTO 606 JAPAN UNIV HAMBURG,DEPT GEN SURG W-2000 HAMBURG 13 GERMANY
Titolo Testata:
Transplantation
fascicolo: 4, volume: 55, anno: 1993,
pagine: 932 - 935
SICI:
0041-1337(1993)55:4<932:DOWLEH>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; RAT SPLEEN; TRANSPLANTATION; IMMUNOSUPPRESSION; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
S. Uyama et al., "DELIVERY OF WHOLE LIVER EQUIVALENT HEPATOCYTE MASS USING POLYMER DEVICES AND HEPATOTROPHIC STIMULATION", Transplantation, 55(4), 1993, pp. 932-935

Abstract

Using hepatocytes injected into prevascularized polymer sponge devices, we studied hepatocyte survival and function after delivery of a whole liver-equivalent of cells into rats. LEW rats and enzyme-deficient Gunn rats served as recipients, respectively. Totally, 28.5 cm2 (0.5-cm thick) of polyvinyl alcohol sponges were implanted per animal. Hepatotrophic stimulation was induced by portacaval shunt and partial (70% or 30%) hepatectomy. Recipient rats received 5 x 10(8) hepatocytes (equivalent to whole rat liver) which were harvested from LEW and Wistar donors, respectively. After engraftment, histologic examination revealed hepatocyte remodeling in the device with capillaries lining plates of hepatocytes, and also tubular structures resembling early biliary radicles. BrdU staining revealed DNA synthesis in hepatocytes, providing evidence of regeneration within the grafts. Quantification of viablehepatocyte area at various time points was performed using computer-assisted morphometry. We then estimated a range of cell numbers from the quantitated cell area. The number of hepatocytes viable at day 7 wasestimated at 27.5-46.0% and 6.6-11.0% in the mesentery and subcutaneous site, respectively. Thus the average number was estimated between 10.8% and 18.0% of initially injected hepatocytes. In the Gunn rat, experiment, experimental rats that received normal Wistar hepatocytes showed a significantly greater decrease in total serum bilirubin comparedwith the concurrent control Gunn rats (P<0.01). At week 1, serum bilirubin in experimental rats decreased to 74.7% (6.80+/-0.16 mg/dl) of pretransplantation level (9.10+/-0.47 mg/dl) and this was 71.4% of the control rats' bilirubin level (9.53+/-0.37 mg/dl). In conclusion, a hepatocyte mass equivalent, to a whole rat liver can be delivered into prevascularized polymer sponge devices. At day 7 between 10.8% and 18.0% of these hepatocytes were estimated to be engrafted and functioning. Further optimization of this technique is necessary before clinical application is considered.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 14:01:46