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Titolo:
FLUOROCHROME-LABELED TYRAMIDES - USE IN IMMUNOCYTOCHEMISTRY AND FLUORESCENCE IN-SITU HYBRIDIZATION
Autore:
VANGIJLSWIJK RPM; ZIJLMANS HJMAA; WIEGANT J; BOBROW MN; ERICKSON TJ; ADLER KE; TANKE HJ; RAAP AK;
Indirizzi:
LEIDEN STATE UNIV,DEPT CYTOCHEM & CYTOMETRY,SYLVIUS LAB,WASSENAARSEWEG 72 NL-2333 AL LEIDEN NETHERLANDS NEN LIFE SCI PROD BOSTON MA 00000
Titolo Testata:
The Journal of histochemistry and cytochemistry
fascicolo: 3, volume: 45, anno: 1997,
pagine: 375 - 382
SICI:
0022-1554(1997)45:3<375:FT-UII>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALKALINE-PHOSPHATASE; SIGNAL AMPLIFICATION; PEROXIDASE; DEPOSITION; BIOTIN;
Keywords:
TSA; FLUORESCENCE; TYRAMIDE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; PEROXIDASE; ANTIBODIES; OLIGONUCLEOTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
R.P.M. Vangijlswijk et al., "FLUOROCHROME-LABELED TYRAMIDES - USE IN IMMUNOCYTOCHEMISTRY AND FLUORESCENCE IN-SITU HYBRIDIZATION", The Journal of histochemistry and cytochemistry, 45(3), 1997, pp. 375-382

Abstract

The peroxidase-mediated deposition of hapten- and fluorochrome-labeled tyramides has recently been shown to increase the sensitivity of immunofluoresence and fluorescence in situ hybridization techniques. We have evaluated a number of red, green, and blue fluorescent tyramides for detection of antigens in tissue sections and cytospin preparations and for the detection of hapten- and horseradish peroxidase-labeled probes hybridized in situ to cells and chromosomes. With few exceptions,all fluorescent tyramide-based methods provided a considerable increase in sensitivity compared to conventional immunofluorescence and FISHmethods.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:21:07