Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHARACTERIZATION OF HEMATOPOIETIC-CELL POPULATIONS FROM HUMAN CORD BLOOD EXPRESSING C-KIT
Autore:
REISBACH G; BARTKE I; KEMPKES B; KOSTKA G; ELLWART J; BIRNER A; THALMEIER K; MAILHAMMER R; BORNKAMM GW; ULLRICH A; DORMER P;
Indirizzi:
GSF INST EXPTL HAMATOL,MARCHIONINISTR 25 W-8000 MUNICH 70 GERMANY MAX PLANCK INST BIOCHEM W-8033 MARTINSRIED GERMANY GSF INST CLIN MOLEC BIOL MUNICH GERMANY
Titolo Testata:
Experimental hematology
fascicolo: 1, volume: 21, anno: 1993,
pagine: 74 - 79
SICI:
0301-472X(1993)21:1<74:COHPFH>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE KINASE RECEPTOR; HUMAN BONE-MARROW; GROWTH-FACTOR; PROGENITOR CELLS; PROTO-ONCOGENE; W-LOCUS; SI-LOCUS; GM-CSF; LIGAND; PRODUCT;
Keywords:
C-KIT EXPRESSION; HUMAN UMBILICAL CORD BLOOD; CFU-GEMM; CD34;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
G. Reisbach et al., "CHARACTERIZATION OF HEMATOPOIETIC-CELL POPULATIONS FROM HUMAN CORD BLOOD EXPRESSING C-KIT", Experimental hematology, 21(1), 1993, pp. 74-79

Abstract

Human cord blood or bone marrow cells expressing the CD34 surface antigen include a population of pluripotent progenitors. We identified and isolated a subpopulation of cells coexpressing CD34 and c-kit, a transmembrane receptor with tyrosine kinase activity. Novel monoclonal antibodies (16A6, 14A3, 3D6) directed against the extracellular domain of c-kit were used for immunofluorescence labeling and sorting of low-density mononuclear cells (MNCs) from umbilical cord blood and bone marrow. The frequency of c-kit-labeled MNCs from cord blood (mean 5.0% +/- 2.1%, n=16) was similar to that from adult bone marrow (mean 3.7% +/- 1.3%, n=4). On average, 1.4% of CD34-positive cells were recorded incord blood and 2.1% in bone marrow MNCs. Roughly 60% of CD34-positivecells coexpressed c-kit. The ability of CD34+/c-kit+ cells to form multilineage colonies (CFU-GEMM) was assayed after sorting with an antibody that did not show any significant effect on c-kit ligand (RL) or granulocyte/macrophage colony-stimulating factor (GM-CSF)-induced colony formation. For CD34+/c-kit+ cells, we found a 20- to 50-fold enrichment as against total MNCs, and a 2-fold enrichment if compared with the CD34+/c-kit- population. To study expression of c-kit in lymphocyticprecursors, monoclonal anti-CD7 or anti-CD10 antibodies were used simultaneously. In contrast to CD34-expressing cells, however, no consistent double-labeled subpopulation of lymphocytic cells was detected. Furthermore, coexpression of CD38 (73% +/- 14%, n=4) or CD33 (29% +/- 12%, n=5) on a majority of c-kit-positive cells showed their lineage commitment to erythropoiesis and granulocytopoiesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 15:40:47