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Titolo:
SUBCELLULAR-LOCALIZATION OF TYPE-I THIONINS IN THE ENDOSPERMS OF WHEAT AND BARLEY
Autore:
CARMONA MJ; HERNANDEZLUCAS C; MARTIN CS; GONZALEZ P; GARCIAOLMEDO F;
Indirizzi:
UNIV POLITECN MADRID,ESCUELA TECN SUPER INGN AGRON,DEPT BIOQUIM,CIUDAD UNIV E-28040 MADRID SPAIN UNIV POLITECN MADRID,ESCUELA TECN SUPER INGN AGRON,DEPT BIOQUIM,CIUDAD UNIV E-28040 MADRID SPAIN UNIV AUTONOMA MADRID,CTR BIOL MOLEC MADRID 34 SPAIN
Titolo Testata:
Protoplasma
fascicolo: 1-2, volume: 173, anno: 1993,
pagine: 1 - 7
SICI:
0033-183X(1993)173:1-2<1:SOTTIT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORDEUM-VULGARE-L; LEAF-SPECIFIC THIONINS; PROTEIN BODY FORMATION; IMMUNOCYTOCHEMICAL LOCALIZATION; BODIES; HORDOTHIONIN; EXPRESSION; GENE;
Keywords:
ENDOSPERM; PROTEIN BODIES; THIONINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
M.J. Carmona et al., "SUBCELLULAR-LOCALIZATION OF TYPE-I THIONINS IN THE ENDOSPERMS OF WHEAT AND BARLEY", Protoplasma, 173(1-2), 1993, pp. 1-7

Abstract

Thionins are cysteine-rich polypeptides of about 5,000 Da. Localization at the subcellular level of type I endosperm thionins has been carried out by immunogold labeling, using an antibody that recognizes typeI thionin variants. In developing wheat and barley caryopses, sectioned at different times between 13 and 24 days after flowering, this type of thionins was only detected around protein bodies from cells of the starchy endosperm, using light microscopy. Electron microscopy revealed that these proteins were located in electron-dense spheroids in the periphery of protein bodies, at the earlier stages, whereas later the label appeared also as a thin layer around these organelles.

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Documento generato il 24/09/20 alle ore 19:32:30