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Titolo:
THE MECHANISM OF INHIBITION OF ALKYLAMINES ON THE MAST-CELL PEPTIDERGIC PATHWAY
Autore:
FISCHER T; BRONNER C; LANDRY Y; MOUSLI M;
Indirizzi:
UNIV LOUIS PASTEUR,NEUROIMMUNOPHARMACOL LAB,BP 24 F-67401 ILLKIRCH GRAFFENS FRANCE UNIV LOUIS PASTEUR,NEUROIMMUNOPHARMACOL LAB,BP 24 F-67401 ILLKIRCH GRAFFENS FRANCE
Titolo Testata:
Biochimica et biophysica acta
fascicolo: 3, volume: 1176, anno: 1993,
pagine: 305 - 312
SICI:
0006-3002(1993)1176:3<305:TMOIOA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUANINE-NUCLEOTIDE-BINDING; PERTUSSIS-TOXIN; G-PROTEINS; POLYPHOSPHOINOSITIDE PHOSPHODIESTERASE; BENZALKONIUM CHLORIDE; HISTAMINE-RELEASE; ACTIVATION; GTP; EXOCYTOSIS; RECEPTOR;
Keywords:
ALKYLAMINE; EXOCYTOSIS; G-PROTEIN; MASTOPARAN; (MAST CELL); (RAT);
Tipo documento:
Article
Natura:
Periodico
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
T. Fischer et al., "THE MECHANISM OF INHIBITION OF ALKYLAMINES ON THE MAST-CELL PEPTIDERGIC PATHWAY", Biochimica et biophysica acta, 1176(3), 1993, pp. 305-312

Abstract

GTP-binding proteins are known to play an important role in controlling mast-cell exocytosis and are described as the primary targets of peptidic mast-cell histamine releasers. The mechanism of inhibition of the mast-cell peptidergic pathway by alkylamines, which are selective inhibitors of this pathway, was investigated using intact or permeabilized rat peritoneal mast cells. Histamine release induced by GTPgammaS and by mastoparan (a venom peptide activating G proteins) was inhibited by pretreating mast cells with 0.1 to 3 mug/ml of a mixture of benzalkonium chloride containing in majority a twelve-carbon-atom aliphaticchain (BAC (C(approximately 12)). Pure benzalkonium chloride, with a fourteen-carbon-atom aliphatic chain (BAC (C-14)), at 5 to 10 muM alsoinhibited histamine release induced by GTPgammaS and mastoparan. The dose-response curve of mastoparan-induced histamine release from intact mast cells was shifted to the right by various concentrations of BAC(C-14). Similar results were obtained with another alkylamine differing from BAC (C-14) by the absence of the benzene ring, tetradecyltrimethylammonium bromide, TAB (C-14). This illustrates that the presence of the phenyl radical is not required for the inhibitory effect of benzalkonium chloride. BAC (C approximately 12) and BAC (C-14) inhibited the generation of inositol polyphosphates induced by GTPgammaS. BAC (C approximately 12) and TAB (C-14) inhibited the mastoparan-stimulated GTPase activity from mast-cell G(i)-like proteins. These results suggest that alkylamines exert selectively their inhibitory effect via an interaction with mast-cell G(i)-like proteins coupled to phospholipase C, i.e., at an early stage in the stimulus-secretion coupling process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 10:02:33