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Titolo:
INHIBITION OF CISPLATIN-MEDIATED DNA DAMAGE INVITRO BY RIBONUCLEOTIDES
Autore:
SEKI S; HONGO A; ZHANG B; AKIYAMA K; SARKER AH; KUDO T;
Indirizzi:
OKAYAMA UNIV,SCH MED,INST CELLULAR & MOLEC BIOL,DEPT MOLEC BIOL,2-5-1SHIKATA CHO OKAYAMA 700 JAPAN OKAYAMA UNIV,SCH MED,DEPT OBSTET & GYNECOL OKAYAMA 700 JAPAN
Titolo Testata:
Japanese journal of cancer research
fascicolo: 4, volume: 84, anno: 1993,
pagine: 462 - 467
SICI:
0910-5050(1993)84:4<462:IOCDDI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II); ACQUIRED-RESISTANCE; PLATINUM COMPLEXES; REPAIR; ADDUCTS; PURINE; CELLS; TRANS-DIAMMINEDICHLOROPLATINUM(II); CIS-DICHLORODIAMMINEPLATINUM(II); MECHANISM;
Keywords:
CISPLATIN; RIBONUCLEOTIDE; DNA DAMAGE; PLATINATION OF NUCLEOTIDE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
S. Seki et al., "INHIBITION OF CISPLATIN-MEDIATED DNA DAMAGE INVITRO BY RIBONUCLEOTIDES", Japanese journal of cancer research, 84(4), 1993, pp. 462-467

Abstract

Effects of ribonucleotides on cis-diamminedichloroplatinum(II) (cisplatin)-mediated DNA damage were studied by incubating pUC18 DNA with cisplatin in the presence of nucleotide, and by monitoring conformational change and sensitivity change to restriction enzyme HpaII of the DNAdue to the platinum-DNA adduct formation. The cisplatin-mediated DNA damage was inhibited in a dose-dependent fashion by ATP or GTP, substantially at their physiological intracellular concentrations, and almost completely by 5 mM ATP or 2 mM GTP. The inhibitory effect of nucleotide on the platination of DNA was in the order of GTP > ATP much greater than CTP > UTP, and of nucleoside triphosphate > nucleoside diphosphate > nucleoside monophosphate. Nucleoside did not show any significant effect on platination of DNA. To elucidate the mechanism of the nucleotide effects on platination of DNA, interaction between ATP or GTP and cisptatin was analyzed by high-performance liquid chromatography. The results suggested that ATP inhibits cisplatin-mediated DNA damage both by forming a platinum-ATP adduct and by non-covalent ionic interaction with cisplatin, while GTP acts largely by forming platinum-GTP adducts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:32:40