Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MODULATION OF Y-79 RETINOBLASTOMA CELL-DIFFERENTIATION AND IRBP EXPRESSION BY DIBUTYRYL-CYCLIC-AMP AND LAMININ
Autore:
FASSINA G; PAGLIALUNGA G; NOONAN DM; CHADER GJ; ALBINI A;
Indirizzi:
IST NAZL RIC CANC,DEPT CHEM CARCINOGENESIS,VIALE BENEDETTO XV 10 I-16132 GENOA ITALY CNR,CTR NEUROFISIOL CEREBRALE I-16132 GENOA ITALY NEI BETHESDA MD 20892
Titolo Testata:
International journal of oncology
fascicolo: 5, volume: 2, anno: 1993,
pagine: 745 - 751
SICI:
1019-6439(1993)2:5<745:MOYRCA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOID-BINDING PROTEIN; ATTACHMENT CULTURE; MESSENGER-RNA; GENE; BUTYRATE; ACID;
Keywords:
RETINOBLASTOMA; DIFFERENTIATION; CYCLIC AMP; LAMININ;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
G. Fassina et al., "MODULATION OF Y-79 RETINOBLASTOMA CELL-DIFFERENTIATION AND IRBP EXPRESSION BY DIBUTYRYL-CYCLIC-AMP AND LAMININ", International journal of oncology, 2(5), 1993, pp. 745-751

Abstract

Y-79 retinoblastoma cells grown on a poly-D-lysine-coated substratum can assume a photoreceptor-like morphology and they express the retina-specific interphotoreceptor-retinoid-binding protein (IRBP) mRNA in abundance. In contrast, cells in suspension culture remain morphologically undifferentiated and exhibit a much lower IRBP message level. After treatment with the differentiating agent dibutyryl cyclic AMP (dbcAMP), monolayer cultures of Y-79 cells grown in serum-containing medium show a partial morphological and biochemical differentiation towards aMuller-glial-like phenotype. This is accompanied by a decrease in IRBP mRNA level with respect to that in Y-79 cells grown on poly-D-lysine. Attached cells which had been pretreated with laminin for three daysdo not demonstrate an altered IRBP mRNA level in response to dbcAMP as compared to cells not pretreated. Surprisingly, however, cells in suspension culture that were exposed to laminin and then to dbcAMP showed a 3-fold increase in IRBP mRNA level. Thus, expression of an important photoreceptor-specific marker is clearly modulated by both dbcAMP and laminin. Moreover, attachment itself may be a significant factor indetermination of a neural or neuro-retinal cell phenotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:14:24