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Titolo:
SIGNAL SEQUENCE REGION OF MITOCHONDRIAL PRECURSOR PROTEINS BINDS TO MITOCHONDRIAL IMPORT RECEPTOR
Autore:
MURAKAMI H; BLOBEL G; PAIN D;
Indirizzi:
ROCKEFELLER UNIV,HOWARD HUGHES MED INST,CELL BIOL LAB NEW YORK NY 10021
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 8, volume: 90, anno: 1993,
pagine: 3358 - 3362
SICI:
0027-8424(1993)90:8<3358:SSROMP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHATE-TRANSPORT PROTEIN; SACCHAROMYCES-CEREVISIAE; CONTACT SITES; TRANSLOCATION; MEMBRANE; IDENTIFICATION; CHLOROPLASTS; CLONING; GENE;
Keywords:
EXPRESSION IN ESCHERICHIA-COLI; CELL-FREE TRANSLATION; PREPROTEINS; INVITRO BINDING; PHOSPHATE TRANSLOCATOR;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
H. Murakami et al., "SIGNAL SEQUENCE REGION OF MITOCHONDRIAL PRECURSOR PROTEINS BINDS TO MITOCHONDRIAL IMPORT RECEPTOR", Proceedings of the National Academy of Sciences of the United Statesof America, 90(8), 1993, pp. 3358-3362

Abstract

An integral mitochondrial membrane protein (p32) of yeast has previously been molecularly cloned and sequenced and suggested to function asa mitochondrial import receptor. However, this protein has also been proposed to function as phosphate translocator [Guerin, B., Bukusoglu,C., Rakotomanana, F. & Wohlrab, H. (1990) J. Biol. Chem. 265, 19736-19741; Phelps, A., Schobert, C. T. & Wohlrab, H. (1991) Biochemistry 30, 248-2521. Here we have purified p32 after expression of its gene in Escherichia coli and assayed its ability to bind to various preproteins containing signal sequences for protein translocation into mitochondria, chloroplasts, or the endoplasmic reticulum. Our data suggest thatp32 contains a binding site specific for the signal sequence region of mitochondrial preproteins. These data are consistent with the previous assignment of p32 as an import receptor and are discussed with regard to the apparently conflicting assignment of this protein as phosphate translocator.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 00:27:47