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Titolo:
T7 RNA-POLYMERASE MUTANTS WITH ALTERED PROMOTER SPECIFICITIES
Autore:
RASKIN CA; DIAZ GA; MCALLISTER WT;
Indirizzi:
SUNY HLTH SCI CTR,MORSE INST MOLEC BIOL & GENET,DEPT MICROBIOL & IMMUNOL,450 CLARKSON AVE BROOKLYN NY 11203 SUNY HLTH SCI CTR,MORSE INST MOLEC BIOL & GENET,DEPT MICROBIOL & IMMUNOL,450 CLARKSON AVE BROOKLYN NY 11203
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 8, volume: 90, anno: 1993,
pagine: 3147 - 3151
SICI:
0027-8424(1993)90:8<3147:TRMWAP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIOPHAGE T3; NUCLEOTIDE-SEQUENCE; X-RAY; GENE; DNA; EXPRESSION; REPRESSOR; PROTEINS; AFFINITY; BINDING;
Keywords:
CLONING VECTORS; PROTEIN-DNA RECOGNITION; DNA-BINDING PROTEINS; HYDROGEN BONDING; PHAGE RNA POLYMERASES;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Raskin et al., "T7 RNA-POLYMERASE MUTANTS WITH ALTERED PROMOTER SPECIFICITIES", Proceedings of the National Academy of Sciences of the United Statesof America, 90(8), 1993, pp. 3147-3151

Abstract

The amino acid at position 748 in T7 RNA polymerase (RNAP) functions to discriminate base pairs at positions -10 and -11 in the promoter. We have constructed a series of T7 RNAP mutants having all possible amino acid substitutions at this position. Surprisingly, most (13/19) substitutions result in active RNAPs, and many of these exhibit altered promoter specificities. Identification of mutant RNAPs with altered specificities expands the repertoire of highly specific phage RNAPs that are available for use in phage RNAP-based transcription systems and highlights the complexity of sequence-specific DNA recognition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 21:57:37