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Titolo:
RADIO-LABEL AND MASS DETERMINATIONS OF INOSITOL 1,3,4,5-TETRAKISPHOSPHATE FORMATION IN RAT CEREBRAL CORTICAL SLICES - DIFFERENTIAL-EFFECTS OF MYOINOSITOL
Autore:
KURIAN P; NARANG N; CHANDLER LJ; CREWS FT;
Indirizzi:
UNIV FLORIDA,COLL MED,DEPT PHARMACOL,BOX 100267 GAINESVILLE FL 32610 UNIV FLORIDA,COLL MED,DEPT PHARMACOL,BOX 100267 GAINESVILLE FL 32610
Titolo Testata:
Neurochemical research
fascicolo: 5, volume: 18, anno: 1993,
pagine: 639 - 645
SICI:
0364-3190(1993)18:5<639:RAMDOI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTEX SLICES; LITHIUM; BRAIN; RECEPTOR; CELLS; ACCUMULATION; METABOLISM; HYDROLYSIS; CA-2+; ASSAY;
Keywords:
BRAIN; CARBACHOL; INOSITOL; INOSITOL PHOSPHATES; INOSITOL TETRAKISPHOSPHATE; LITHIUM;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
P. Kurian et al., "RADIO-LABEL AND MASS DETERMINATIONS OF INOSITOL 1,3,4,5-TETRAKISPHOSPHATE FORMATION IN RAT CEREBRAL CORTICAL SLICES - DIFFERENTIAL-EFFECTS OF MYOINOSITOL", Neurochemical research, 18(5), 1993, pp. 639-645

Abstract

To investigate the effects of increasing concentrations of myo-inositol (inositol) on receptor stimulated [H-3]inositol polyphosphate formation in the absence of lithium, slices of rat cerebral cortex were incubated with various concentrations of [H-3]inositol (1 to 30 muM). Carbachol stimulated formation of [H-3]inositol trisphosphate (InsP3) and[H-3]inositol 1,3,4,5-tetrakisphosphate {Ins(1,3,4,5)P4} increased several fold when the inositol concentration was increased reaching a plateau at approximately 12 muM inositol. Time course studies revealed that in the presence of low concentrations of inositol (1 muM), [H-3]InsP, and [H-3]Ins(1,3,4,5)P4 formation in response to carbachol stimulation increased slowly over a 10 to 20 min time period, whereas in the presence of 4 and 12 muM inositol, carbachol stimulated [H-3]InsP3 and[H-3]Ins(1,3,4,5)P, formation was rapid and essentially complete within 3 to 5 min after carbachol addition. Although the carbachol dose response in 12 muM inositol had a much greater maximal efficacy, there was no change in potency. Similar to the effects of carbachol on [H-3]Ins(1,3,4,5)P4 formation from prelabeled phosphoinositides, muscarinic receptor stimulation increased Ins(1,3,4,5)P4 mass formation by seven fold. Furthermore, Li+ (8 mM) completely inhibited carbachol stimulated increases in Ins(1,3,4,5)P, mass formation. In contrast to the effects of increasing inositol on carbachol stimulated formation of radiolabeled inositol phosphates, increasing inositol had no effect upon massformation of Ins(1,3,4,5)P4. These results show that when measuring inositol polyphosphate formation by the radiolabeling technique in the absence of Li+, increasing the inositol concentration greatly increases the stimulated component of [H-3]InsP3 and [H-3]Ins(1,3,4,5)P4 formation. However, this inositol induced increase in agonist stimulated Ins(1,3,4,5)P4 formation is not reflected as an increase in mass formation.

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Documento generato il 02/12/20 alle ore 16:37:26