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Titolo:
DETERMINATION OF IODOFORM IN RABBIT PLASMA BY HPLC
Autore:
NAGATA E; YASUDA S; KASAHARA T; KUBO H; MIURA S;
Indirizzi:
KITASATO UNIV,E HOSP,DIV RES,2-1-1 ASAMIZODAI SAGAMIHARA KANAGAWA 228JAPAN KITASATO UNIV,SCH MED,DEPT PSYCHIAT SAGAMIHARA KANAGAWA 228 JAPAN KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU TOKYO 108 JAPAN
Titolo Testata:
Bunseki Kagaku
fascicolo: 4, volume: 42, anno: 1993,
pagine: 215 - 218
SICI:
0525-1931(1993)42:4<215:DOIIRP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
JPN
Keywords:
PLASMA IODOFORM CONCENTRATION; HPLC; IODOFORM INTOXICATION; DRUG MONITORING; PLASMA OF RABBIT;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
3
Recensione:
Indirizzi per estratti:
Citazione:
E. Nagata et al., "DETERMINATION OF IODOFORM IN RABBIT PLASMA BY HPLC", Bunseki Kagaku, 42(4), 1993, pp. 215-218

Abstract

A simple, rapid and reproducible method for the determination of iodoform in rabbit plasma by HPLC has been developed. Under the light-resistant condition, iodoform and diiodomethane (as internal standard) in 1.0 ml plasma were extracted with 1.0 ml diethylether. Twenty microliters of the organic phase were injected into the HPLC system, consistedof a Model 510, U6K-injector, Model 481 UV detector setting at 336 nm(0.005 AUFS), an Ultrasphere ODS 5 mum guard column (45 mm X 4.6 mm i.d.) and an analytical column (150 mm X 4.6 mm i.d.). The mobile phasewas 60% methanol/pH 3.8, 5 mM potassium phosphate buffer. The flow rate was 1.0 ml/min. The retention time for iodoform and diiodomethane were 10.4 and 7.7 min, respectively. The limit of detection was 0.3 mug/ml(S/N = 3) of plasma. Within-run reproducibility for 0.4 mug/ml was /- 2.1 % (n = 9). The HPLC separation can be completed in less than 11 min and had been applied for plasma of rabbit packed intraperitoneally by iodoform gauze.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:58:29