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Titolo:
THE BIOCHEMICAL DEFECTS OF PRP4-1 AND PRP6-1 YEAST SPLICING MUTANTS REVEAL THAT THE PRP6 PROTEIN IS REQUIRED FOR THE ACCUMULATION OF THE [U4 U6.U5] TRI-SNRNP/
Autore:
GALISSON F; LEGRAIN P;
Indirizzi:
INST PASTEUR,DEPT BIOL MOLEC,UNITE GENET MOLEC LEVURES,28 RUE DR ROUXF-75724 PARIS 15 FRANCE INST PASTEUR,DEPT BIOL MOLEC,UNITE GENET MOLEC LEVURES,28 RUE DR ROUXF-75724 PARIS 15 FRANCE
Titolo Testata:
Nucleic acids research
fascicolo: 7, volume: 21, anno: 1993,
pagine: 1555 - 1562
SICI:
0305-1048(1993)21:7<1555:TBDOPA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; SMALL NUCLEAR RIBONUCLEOPROTEIN; SACCHAROMYCES-CEREVISIAE; U1 SNRNA; SPLICEOSOME; PATHWAY; BINDING; GENES; IDENTIFICATION; COMPLEXES;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
F. Galisson e P. Legrain, "THE BIOCHEMICAL DEFECTS OF PRP4-1 AND PRP6-1 YEAST SPLICING MUTANTS REVEAL THAT THE PRP6 PROTEIN IS REQUIRED FOR THE ACCUMULATION OF THE [U4 U6.U5] TRI-SNRNP/", Nucleic acids research, 21(7), 1993, pp. 1555-1562

Abstract

We have raised specific antibodies against the PRP6 protein and shownthat the U4, U5 and U6 snRNAs are co-precipitated with this protein. Using splicing extracts prepared from in vivo heat-inactivated cells, we have characterized the prp4-1 and prp6-1 biochemical defects. In inactivated prp4-1 cell extracts, the U6 snRNA content as well as the U6, U4/U6 snRNPs and the [U4/U6.U5] tri-snRNP particles amounts are severely reduced. In inactivated prp6-1 cell extracts, the PRP6 mutant protein is barely detectable. Glycerol gradient analyses indicate that, in these extracts, the [U4/U6.U5] tri-snRNPs are present in very low amounts, but U4/U6 snRNP particles are normally represented. These results establish that the PRP6 protein is required for the accumulation ofthe [U4/U6.U5] tri-snRNP. We found no evidence for the presence of the PRP6 protein in the U4/U6 particle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:22:39