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Titolo:
FUNCTIONAL DIVERGENCE OF PROTEIN-KINASE-C (PKC) FAMILY MEMBERS - PKC-GAMMA DIFFERS FROM PKC-ALPHA AND PKC-BETA-II AND NPKC-EPSILON IN ITS COMPETENCE TO MEDIATE-12-O-TETRADECANOYL PHORBOL 13-ACETATE (TPA)-RESPONSIVE TRANSCRIPTIONAL ACTIVATION THROUGH A TPA-RESPONSE ELEMENT
Autore:
HATA A; AKITA Y; SUZUKI K; OHNO S;
Indirizzi:
YOKOHAMA CITY UNIV,SCH MED,DEPT MOLEC BIOL,3-9 FUKU URA,KANAZAWA KU YOKOHAMA 236 JAPAN TOKYO METROPOLITAN INST MED SCI,DEPT MOLEC BIOL,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 12, volume: 268, anno: 1993,
pagine: 9122 - 9129
SICI:
0021-9258(1993)268:12<9122:FDOP(F>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING ACTIVITY; FOS GENE; DISTINCT FORMS; SERUM FACTORS; RAT-BRAIN; EXPRESSION; JUN; RECEPTOR; ONCOGENE; AP-1;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
A. Hata et al., "FUNCTIONAL DIVERGENCE OF PROTEIN-KINASE-C (PKC) FAMILY MEMBERS - PKC-GAMMA DIFFERS FROM PKC-ALPHA AND PKC-BETA-II AND NPKC-EPSILON IN ITS COMPETENCE TO MEDIATE-12-O-TETRADECANOYL PHORBOL 13-ACETATE (TPA)-RESPONSIVE TRANSCRIPTIONAL ACTIVATION THROUGH A TPA-RESPONSE ELEMENT", The Journal of biological chemistry, 268(12), 1993, pp. 9122-9129

Abstract

We have established an assay system where overexpression of a specific protein kinase C (PKC) type caused by introduction of the respectivecDNA results in the enhancement of a cell response: the transcriptional activation of a set of genes in response to PKC activators such as 12-O-tetradecanoylphorbol 13-acetate (TPA). When monitored by the expression of a reporter gene containing the chloramphenicol acetyltransferase gene fused downstream of a synthetic TPA response element (TRE) or a serum response element (SRE), the overexpression of cPKCalpha and betaII or nPKCepsilon all resulted in the enhancement of transcriptional activation through both TRE and SRE. On the other hand, PKCgamma activates TRE only very weakly, although it activates SRE in a similar manner to the other PKC members examined. The overexpression of cPKCalpha and -betaII or nPKCepsilon, but not cPKCgamma, resulted in the enhanced expression of the endogenous c-jun gene, which contains TRE in the 5'-upstream, promoter region. The gel mobility shift assay showed that the activation of PKCgamma, as well as PKCalpha and -betaII and nPKCepsilon, causes the increase in TRE-binding proteins, suggesting thattranscriptional activation through TRE requires an additional step, which is not activated by PKCgamma, such as a qualitative change in TRE-binding or in TRE-associating proteins. This finding provides not only a rationale to explain the presence of multiple PKC family members, but also permits the dissection of the complex cellular signaling cascade involving PKC family members.

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Documento generato il 20/01/21 alle ore 15:28:43