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Titolo:
AUTOPHOSPHORYLATION OF INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS
Autore:
FERRIS CD; CAMERON AM; BREDT DS; HUGANIR RL; SNYDER SH;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOLEC SCI BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT & BEHAV SCI BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,HOWARD HUGHES MED INST BALTIMORE MD 21205
Titolo Testata:
The Journal of biological chemistry
fascicolo: 10, volume: 267, anno: 1992,
pagine: 7036 - 7041
SICI:
0021-9258(1992)267:10<7036:AOI1R>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE SARCOPLASMIC-RETICULUM; RECONSTITUTED LIPID VESICLES; CALCIUM-RELEASE CHANNEL; PROTEIN-KINASE-C; TRISPHOSPHATE RECEPTOR; SKELETAL-MUSCLE; RYANODINE RECEPTOR; ENDOPLASMIC-RETICULUM; MEMBRANE-PROTEIN; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
C.D. Ferris et al., "AUTOPHOSPHORYLATION OF INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS", The Journal of biological chemistry, 267(10), 1992, pp. 7036-7041

Abstract

Inositol 1,4,5-trisphosphate (IP3) releases internal stores of calcium by binding to a specific membrane receptor which includes both the IP3 recognition site as well as the associated calcium channel. The IP3receptor is regulated by ATP, calcium, and phosphorylation by proteinkinase A, protein kinase C, and calcium/calmodulin-dependent protein kinase II. Its cDNA sequence predicts at least two consensus sequenceswhere nucleotides might bind, and direct binding of ATP to the IP3 receptor has been demonstrated. In the present study, we demonstrate autophosphorylation of the purified and reconstituted IP3 receptor on serine and find serine protein kinase activity of the IP3 receptor towarda specific peptide substrate. Several independent purification procedures do not separate the IP3 receptor protein from the phosphorylatingactivity, and many different protein kinase activators and inhibitorsdo not identify protein kinases as contaminants. Also, renaturation experiments reveal autophosphorylation of the monomeric receptor on polyvinylidene difluoride membranes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 04:49:08