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Titolo:
ISOLATION AND CHARACTERIZATION OF SFP - A GENE THAT FUNCTIONS IN THE PRODUCTION OF THE LIPOPEPTIDE BIOSURFACTANT, SURFACTIN, IN BACILLUS-SUBTILIS
Autore:
NAKANO MM; CORBELL N; BESSON J; ZUBER P;
Indirizzi:
LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL SHREVEPORT LA 71130
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 2, volume: 232, anno: 1992,
pagine: 313 - 321
SICI:
0026-8925(1992)232:2<313:IACOS->2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SECONDARY METABOLITES; PEPTIDE ANTIBIOTICS; BIOSYNTHESIS; CLONING; COMPETENCE; PROMOTER;
Keywords:
SURFACTIN; LIPOPEPTIDE ANTIBIOTIC; BACILLUS-SUBTILIS; SFP; SRFA;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M.M. Nakano et al., "ISOLATION AND CHARACTERIZATION OF SFP - A GENE THAT FUNCTIONS IN THE PRODUCTION OF THE LIPOPEPTIDE BIOSURFACTANT, SURFACTIN, IN BACILLUS-SUBTILIS", MGG. Molecular & general genetics, 232(2), 1992, pp. 313-321

Abstract

The sfp gene is required for cells of Bacillus subtilis to become producers of the lipopeptide antibiotic surfactin. sfp was isolated and its nucleotide sequence was determined. sfp was expressed in Escherichia coli and its putative product was purified for use in antibody production and in amino acid sequence analysis. The gene was plasmid-amplified in B. subtilis, where it conferred a Srf+ phenotype on sfp0 (surfactin non-producing) cells. Overproduction of Sfp in B. subtilis did not cause production of an increased amount of surfactin and resulted inthe repression of a lacZ transcriptional fusion of the srfA operon, which encodes enzymes that catalyze surfactin synthesis. We propose that sfp represents an essential component of peptide synthesis systems and also plays a role, either directly or indirectly, in the regulationof surfactin biosynthesis gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 03:40:22