Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIFFERENTIAL EXPRESSION OF THE ALPHA-2 AND BETA MESSENGER-RNAS OF NA,K-ATPASE IN DEVELOPING BRINE SHRIMP AS MEASURED BY INSITU HYBRIDIZATION
Autore:
SUN DY; GUO JZ; HARTMANN HA; UNO H; HOKIN LE;
Indirizzi:
UNIV WISCONSIN,SCH MED,DEPT PATHOL & LAB MED MADISON WI 53706 UNIV WISCONSIN,DEPT PHARMACOL MADISON WI 53706 UNIV WISCONSIN,WISCONSIN REG PRIMATE RES CTR MADISON WI 53706
Titolo Testata:
The Journal of histochemistry and cytochemistry
fascicolo: 4, volume: 40, anno: 1992,
pagine: 555 - 562
SICI:
0022-1554(1992)40:4<555:DEOTAA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
K+)-ACTIVATED ADENOSINE-TRIPHOSPHATASE; ARTEMIA-SALINA NAUPLII; MOLECULAR-CLONING; SODIUM-PUMP; AMINO-ACID; SUBUNIT; BIOSYNTHESIS; PURIFICATION; ATPASE;
Keywords:
NA,K-ATPASE; MESSENGER RNA INSITU HYBRIDIZATION; BRINE SHRIMP; ARTEMIA; DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
D.Y. Sun et al., "DIFFERENTIAL EXPRESSION OF THE ALPHA-2 AND BETA MESSENGER-RNAS OF NA,K-ATPASE IN DEVELOPING BRINE SHRIMP AS MEASURED BY INSITU HYBRIDIZATION", The Journal of histochemistry and cytochemistry, 40(4), 1992, pp. 555-562

Abstract

We used in situ hybridization histochemistry with synthetic oligonucleotide probes to localize the mRNAs encoding the alpha(2)- and beta-mRNAs of Na,K-ATPase during development of the brine shrimp Artemia. ThemRNAs of the alpha(2)- and beta-subunit were of low abundance in the cysts; in addition, less mRNA of the beta-subunit was localized. During emergence (12 hr), there was an increase in alpha(2)-subunit mRNA inthe gut mucosa, but there was a burst in beta-subunit mRNA throughout. As development progressed, the mRNAs of both the alpha(2)- and beta-subunits showed a distinct pattern of expression in which the mRNA in the salt gland was of greatest abundance, followed by epidermal cells and gut mucosa. After 36 hr the alpha(2)-subunit mRNA began to decrease in all positive cells but still remained highest in the salt gland and the brain region, while the mRNA of the beta-subunit kept increasing in the gut mucosa. Finally, the greatest abundance of the beta-subunit mRNA shifted from the salt gland to the antenna gland and the epidermal cells in the tail region, but the alpha(2)-subunit mRNA did not. The more widespread distribution of the beta-mRNA than alpha(2)-mRNA at certain stages (e.g., there was no alpha(2)-mRNA in the antenna gland at the adult stage) is in all likelihood due to the marked drop in the alpha(2)-subunit and a rise in alpha(1)-subunit previously seen by Peterson et al. on polyacrylamide gel electrophoresis, as development progresses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 23:53:54