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Titolo:
DIFFERENTIAL-EFFECTS OF ESTROGEN AND ANTIESTROGEN ON TRANSFORMING GROWTH-FACTOR GENE-EXPRESSION IN ENDOMETRIAL ADENOCARCINOMA CELLS
Autore:
GONG YW; BALLEJO G; MURPHY LC; MURPHY LJ;
Indirizzi:
UNIV MANITOBA,FAC MED,DEPT INTERNAL MED,ROOM 435 BASIC SCI BLDG,770 BANNATYNE AVE WINNIPEG R3E 0W3 MANITOBA CANADA UNIV MANITOBA,FAC MED,DEPT PHYSIOL WINNIPEG R3E 0W3 MANITOBA CANADA UNIV MANITOBA,FAC MED,DEPT BIOCHEM WINNIPEG R3E 0W3 MANITOBA CANADA
Titolo Testata:
Cancer research
fascicolo: 7, volume: 52, anno: 1992,
pagine: 1704 - 1709
SICI:
0008-5472(1992)52:7<1704:DOEAAO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BREAST CANCER; MESSENGER RIBONUCLEIC-ACID; ISHIKAWA LINE; FACTOR-ALPHA; ESTRADIOL; PROLIFERATION; 4-HYDROXYTAMOXIFEN; ANTIOESTROGENS; TAMOXIFEN;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
Y.W. Gong et al., "DIFFERENTIAL-EFFECTS OF ESTROGEN AND ANTIESTROGEN ON TRANSFORMING GROWTH-FACTOR GENE-EXPRESSION IN ENDOMETRIAL ADENOCARCINOMA CELLS", Cancer research, 52(7), 1992, pp. 1704-1709

Abstract

While antiestrogens are useful agents in the treatment of breast cancer, the usefulness of these agents in the treatment of endometrial cancer remains controversial. There is some concern that the currently available antiestrogens may have partial agonist activity in uterine tissue. To better understand the mechanisms by which estrogens and antiestrogens modulate growth of endometrial adenocarcinoma cells, we have compared the effects of 17-beta estradiol and three antiestrogens, 4-hydroxytamoxifen (OH-TAM), ICI 164384, and LY 117018 on proliferation and transforming growth factor (TGF) mRNA accumulation in two human endometrial adenocarcinoma cell lines. In HEC-50 cells, neither estradiol nor antiestrogens had any effect on cell proliferation or TGF mRNA abundance under estrogen-depleted culture conditions [basal medium containing 1% twice charcoal-treated fetal bovine serum (ctFBS)] or in the presence of estrogen (basal medium containing 5% fetal bovine serum). At very high concentrations, both estradiol and OH-TAM caused a small decrease in HEC-50 cell proliferation in medium containing 5% serum. Incontrast, the antiestrogens had different effects on Ishikawa cells, depending upon the culture conditions. In medium containing 5% fetal bovine serum, the antiestrogens inhibited cell proliferation and significantly decreased TGF-alpha mRNA abundance and TGF-alpha secretion. OH-TAM was more potent than the other antiestrogens. Under these cultureconditions, estradiol had no effect on cell proliferation or TGF-alpha mRNA levels but increased TGF-alpha secretion. In medium supplemented with 1% ctFBS, estradiol increased cell proliferation and TGF-alpha mRNA (2.72-fold, P < 0.005) and TGF-alpha secretion (700 +/- 156 versus 250 +/- 23 pg/10(6) cells/24 h, P < 0.05), whereas OH-TAM, which also stimulated cell proliferation, reduced TGF-alpha mRNA abundance (P <0.05) but had no significant effect on TGF-alpha secretion. Under these conditions, ICI 164384 and LY 117018 had no effect on either cell proliferation or TGF-alpha expression. Estradiol treatment decreased, whereas OH-TAM increased, epidermal growth factor receptors in Ishikawacells. Both estradiol and the antiestrogens decreased TGF-beta-1 mRNAabundance when cells were grown in media containing 1% ctFBS. In summary, the response of human endometrial adenocarcinoma cells to estrogen and antiestrogens varied between cell lines and was dependent upon the culture conditions used. In addition, OH-TAM, unlike the other two antiestrogens tested, had growth-stimulating effects on Ishikawa cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 12:11:55