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Titolo:
EVIDENCE FOR INTRARENAL KALLIKREIN STORAGE DURING CHROMATE-INDUCED ACUTE-RENAL-FAILURE IN RAT
Autore:
ORFILA C; SUC JM; GIROLAMI JP;
Indirizzi:
FAC MED RANGUEIL,INSERM,U133,133 ROUTE NARBONNE F-31062 PARIS FRANCE
Titolo Testata:
Histochemistry
fascicolo: 3, volume: 97, anno: 1992,
pagine: 293 - 301
SICI:
0301-5564(1992)97:3<293:EFIKSD>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
URINARY KALLIKREIN; MERCURIC-CHLORIDE; CORTICAL SLICES; PROXIMAL TUBULE; KININ SYSTEM; LOCALIZATION; EXCRETION; EXPOSURE; NEPHRON; MARKER;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
C. Orfila et al., "EVIDENCE FOR INTRARENAL KALLIKREIN STORAGE DURING CHROMATE-INDUCED ACUTE-RENAL-FAILURE IN RAT", Histochemistry, 97(3), 1992, pp. 293-301

Abstract

During the course of chromate-induced acute renal failure (ARF), urinary kallikrein excretion (UKE), a serine protease of distal tubule origin in the normal animal was decreased but tissue kallikrein concentration (TK) was increased, suggesting intracellular accumulation. Severemorphological lesions were observed in proximal tubular cells which showed brush border damage, numerous vesicles, necrosis and liquefaction of cytoplasmic material. Less marked changes were also present in distal tubules: large apical vacuoles and swollen mitochondria. Comparedto normal rats, using the peroxidase-anti-peroxidase (PAP) method forlight microscopy, greater kallikrein immunoreactivity was detected along the apical pole in distal tubules, on the membrane and in the cytoplasm as well as in the glomerulus. By immunoelectron microscopy, kallikrein was found in the connecting apical area, along the luminal, basolateral and basement membranes, in some vesicles, in Golgi apparatus and on ribosomes bound to endoplasmic reticulum. In the glomerulus, kallikrein was observed along the luminal surface of endothelial cell. After 14 days a progressive recovery of renal function, tissue morphology and UKE towards control values was observed. The presence of immunoreactive kallikrein in the glomerulus observed only during ARF confirmed the previous demonstration of kallikrein mRNA in the glomerulus. The cellular accumulation results more likely from a dysfunction of a general secretory mechanism due to cell membrane alteration than from a specific inhibition of kallikrein production and secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 23:40:35