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Titolo:
HUMAN SKIN AS TARGET FOR ALDOSTERONE - COEXPRESSION OF MINERALOCORTICOID RECEPTORS AND 11-BETA-HYDROXYSTEROID DEHYDROGENASE
Autore:
KENOUCH S; LOMBES M; DELAHAYE F; EUGENE E; BONVALET JP; FARMAN N;
Indirizzi:
UNIV PARIS 07,INSERM,U246,16 RUE HENRI HUCHARD,BP416 F-75870 PARIS 18FRANCE UNIV PARIS 07,INSERM,U246 F-75870 PARIS 18 FRANCE INSERM,U33,HORMONES LAB F-94276 LE KREMLIN BICETR FRANCE
Titolo Testata:
The Journal of clinical endocrinology and metabolism
fascicolo: 5, volume: 79, anno: 1994,
pagine: 1334 - 1341
SICI:
0021-972X(1994)79:5<1334:HSATFA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-IDIOTYPIC ANTIBODY; NA+-K+-ATPASE; IMMUNOHISTOCHEMICAL LOCALIZATION; DISTAL NEPHRON; INTERNAL IMAGE; RAT-KIDNEY; PHYSIOLOGY; ISOFORMS; BINDING; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
S. Kenouch et al., "HUMAN SKIN AS TARGET FOR ALDOSTERONE - COEXPRESSION OF MINERALOCORTICOID RECEPTORS AND 11-BETA-HYDROXYSTEROID DEHYDROGENASE", The Journal of clinical endocrinology and metabolism, 79(5), 1994, pp. 1334-1341

Abstract

The expression of mineralocorticoid receptors (MR) and 11 beta-hydroxysteroid dehydrogenase (11HSD) activity has been investigated in the epidermis and appendages of the human skin. Aldosterone binds to MR andregulates sodium transport in tight epithelia. Mineralocorticoid selectivity is achieved through coexpression of MR and 11HSD, which prevents permanent MR occupancy by glucocorticoids. Some forms of hypertension may involve abnormalities of MR and/or 11HSD. However, their directassessment in humans remains difficult in the kidney or colon. This led us to explore this system in human skin easily accessible to biopsy. In situ hybridization with specific MR complementary ribonucleic acid probes and immunohistochemistry using three different anti-MR antibodies showed that MR was expressed at both the messenger ribonucleic acid and protein levels in the keratinocytes of the epidermis, in the sweat and sebaceous glands, and in the hair follicles. A significant 11HSD activity was found in isolated sweat gland ducts (5 fmol/3-mm length.10-min incubation with 10 nmol/L corticosterone as substrate) and was very low in the epidermis. In both structures, reductase activity was 10 times lower than that of dehydrogenase. Studies on the cofactor specificity of the enzyme showed a nicotinamide-adenine-dinucleotide preference in sweat glands, contrasting with a nicotinamide-adenine-dinucleotide phosphate dependence in epidermis. Human skin appears as a new target for aldosterone because it coexpresses MR and 11HSD. Our findings present the possibility to explore the functionality of the MR system in human tissue and its implications in various physiopathological. situations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:27:28