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Titolo:
MYRISTATE EXCHANGE - A 2ND GLYCOSYL PHOSPHATIDYLINOSITOL MYRISTOYLATION REACTION IN AFRICAN TRYPANOSOMES
Autore:
BUXBAUM LU; RAPER J; OPPERDOES FR; ENGLUND PT;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205 INT INST CELLULAR & MOLEC PATHOL,TROP DIS RES UNIT B-1200 BRUSSELS BELGIUM
Titolo Testata:
The Journal of biological chemistry
fascicolo: 48, volume: 269, anno: 1994,
pagine: 30212 - 30220
SICI:
0021-9258(1994)269:48<30212:ME-A2G>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
VARIANT SURFACE GLYCOPROTEIN; HEPATIC MONOACYLGLYCEROL ACYLTRANSFERASE; MEMBRANE ANCHOR; SACCHAROMYCES-CEREVISIAE; PHOSPHOLIPASE-C; BRUCEI; BIOSYNTHESIS; ACYL; SPECIFICITY; BILAYER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
L.U. Buxbaum et al., "MYRISTATE EXCHANGE - A 2ND GLYCOSYL PHOSPHATIDYLINOSITOL MYRISTOYLATION REACTION IN AFRICAN TRYPANOSOMES", The Journal of biological chemistry, 269(48), 1994, pp. 30212-30220

Abstract

The variant surface glycoprotein of African trypanosomes has a glycosyl phosphatidylinositol (GPI) anchor that is unusual in that its fattyacids are exclusively myristate. We showed previously that the myristate is added to a free GPI in a fatty acid remodeling reaction involving deacylation and reacylation, forming glycolipid A, the anchor precursor. We now demonstrate that trypanosomes have a second pathway for GPI anchor myristoylation distinct from the fatty acid remodeling pathway, which we call ''myristate exchange. '' This reaction involves exchange of myristate into both the sn-1 and sn-2 positions of glycolipid A, which already contain myristate. Myristoyl-CoA, the probable myristate donor in the exchange reaction, has an apparent K-m of about 6 nM. We have now identified a lyso-GPI, named theta', which has myristate as its sole fatty acid; the kinetics of formation and utilization of theta' are consistent with it being an intermediate in exchange. Myristate exchange and fatty acid remodeling appear to occur in different subcellular compartments, and the two reactions have different sensitivities to inhibitors. The myristate exchange reaction may be a proofreading system to ensure that the fatty acids on variant surface glycoproteins are exclusively myristate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 09:10:04