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Titolo:
MOLECULAR CHARACTERIZATION OF A NOVEL TOBACCO PATHOGENESIS-RELATED (PR) PROTEIN - A NEW PLANT CHITINASE LYSOZYME/
Autore:
HEITZ T; SEGOND S; KAUFFMANN S; GEOFFROY P; PRASAD V; BRUNNER F; FRITIG B; LEGRAND M;
Indirizzi:
UNIV STRASBOURG 1,CNRS,INST BIOL MOLEC PLANTES,12 RUE GEN ZIMMER F-67000 STRASBOURG FRANCE UNIV STRASBOURG 1,CNRS,INST BIOL MOLEC PLANTES F-67000 STRASBOURG FRANCE
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 2, volume: 245, anno: 1994,
pagine: 246 - 254
SICI:
0026-8925(1994)245:2<246:MCOANT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA ACCUMULATION; BACILLUS-CIRCULANS WL-12; AFFINITY-CHROMATOGRAPHY; SERRATIA-MARCESCENS; BIOLOGICAL FUNCTION; LYSOZYME CHITINASE; ANTIFUNGAL; SEQUENCE; ENZYME; GENE;
Keywords:
NICOTIANA TABACUM; PATHOGENESIS-RELATED PROTEIN; CHITINASE; GENE EXPRESSION; VACUOLAR TARGETING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
T. Heitz et al., "MOLECULAR CHARACTERIZATION OF A NOVEL TOBACCO PATHOGENESIS-RELATED (PR) PROTEIN - A NEW PLANT CHITINASE LYSOZYME/", MGG. Molecular & general genetics, 245(2), 1994, pp. 246-254

Abstract

A new PR (pathogenesis-related) protein was isolated from tobacco leaves (Nicotiana tabacum cv. Samsun NN), reacting hypersensitively to tobacco mosaic virus (TMV), by zinc chelate chromatography and was therefore named Pz. Its reactivity toward several lectins indicated the presence of bound sugar residues. From the amino acid sequence of trypticpeptides, Oligonucleotide primers were derived which allowed the synthesis of Pz cDNA by PCR. Using this cDNA as probe, near full-length clones were isolated from a library made from poly(A)+ RNA purified fromTMV-infected leaves. Sequence analysis revealed similarities with chitinases/lysozymes of various origins and the purified protein was, indeed, shown to hydrolyse different N-acetylglucosamine-containing substrates. Comparison of peptide and cDNA sequences indicated that Pz protein is synthesized as a pre-pro-protein, a seven-amino acid C-terminalpeptide probably being involved in the vacuolar targeting of the protein. Pz mRNA and protein were demonstrated to accumulate strongly in TMV-infected tobacco leaves. Pz transcripts were also found in various tissues of healthy plants, indicating that Pz gene expression is developmentally regulated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:04:24