Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CLONING OF AN INSECTICIDAL CHOLESTEROL OXIDASE GENE AND ITS EXPRESSION IN BACTERIA AND IN PLANT-PROTOPLASTS
Autore:
CORBIN DR; GREENPLATE JT; WONG EY; PURCELL JP;
Indirizzi:
MONSANTO CO,AGR GRP,700 CHESTERFIELD VILLAGE PKWY ST LOUIS MO 63198
Titolo Testata:
Applied and environmental microbiology
fascicolo: 12, volume: 60, anno: 1994,
pagine: 4239 - 4244
SICI:
0099-2240(1994)60:12<4239:COAICO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREVIBACTERIUM-STEROLICUM; ESCHERICHIA-COLI; 3BETA-HYDROXYSTEROID OXIDASE; STREPTOMYCES-LIVIDANS; IMPORTANT INSECTS; PURIFICATION; PROTEINS; SEQUENCE; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
D.R. Corbin et al., "CLONING OF AN INSECTICIDAL CHOLESTEROL OXIDASE GENE AND ITS EXPRESSION IN BACTERIA AND IN PLANT-PROTOPLASTS", Applied and environmental microbiology, 60(12), 1994, pp. 4239-4244

Abstract

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid protein whose first 43 amino acids constitute a secretory signal peptide. Expression of the gene with the signal sequence in Escherichia. coil resulted in production of a protein that had enzymatic and insecticidal properties which were indistinguishable from those of the cholesterol oxidase secreted by Streptomyces sp. strain A19249. Expression ofthe gene with or without the signal sequence in tobacco protoplasts resulted in production of an enzymatically active cholesterol oxidase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:16:47