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Titolo:
MOLECULAR-CLONING AND GENE-EXPRESSION DURING THE HYPERSENSITIVE REACTION TO TOBACCO MOSAIC-VIRUS AND THE RESPONSE TO A FUNGAL ELICITOR
Autore:
PELLEGRINI L; ROHFRITSCH O; FRITIG B; LEGRAND M;
Indirizzi:
CARNEGIE INST WASHINGTON,DEPT EMBRYOL,115 W UNIV PKWY BALTIMORE MD 21210 UNIV STRASBOURG 1,INST BIOL MOLEC PLANTES,CNRS F-67084 STRASBOURG FRANCE
Titolo Testata:
Plant physiology
fascicolo: 3, volume: 106, anno: 1994,
pagine: 877 - 886
SICI:
0032-0889(1994)106:3<877:MAGDTH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENYLALANINE AMMONIA-LYASE; CELL-SUSPENSION CULTURES; MEDICAGO-SATIVA L; PLANT DEFENSE GENES; STRESS RESPONSES; SALICYLIC-ACID; PHENYLPROPANOID METABOLISM; DIFFERENTIAL INDUCTION; ACQUIRED-RESISTANCE; TRANSIENT INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
L. Pellegrini et al., "MOLECULAR-CLONING AND GENE-EXPRESSION DURING THE HYPERSENSITIVE REACTION TO TOBACCO MOSAIC-VIRUS AND THE RESPONSE TO A FUNGAL ELICITOR", Plant physiology, 106(3), 1994, pp. 877-886

Abstract

A tobacco (Nicotiana tabacum L. cv Samsun NN) cDNA clone coding the enzyme phenylalanine ammonia-lyase (PAL) was isolated from a cDNA library made from polyadenylated RNA purified from tobacco mosaic virus (TMV)-infected leaves. Southern analysis indicated that, in tobacco, PAL is encoded by a small family of two to four unclustered genes. Northern analysis showed that PAL genes are weakly expressed under normal physiological conditions, they are moderately and transiently expressed after wounding, but they are strongly induced during the hypersensitivereaction to TMV or to a fungal elicitor. Ribonuclease protection experiments confirmed this evidence and showed the occurrence of two highly homologous PAL messengers originating from a single gene or from twotightly co-regulated genes. By in situ RNA-RNA hybridization PAL transcripts were shown to accumulate in a narrow zone of leaf tissue surrounding necrotic lesions caused by TMV infection or treatment with the fungal elicitor. In this zone, no cell specificity was observed and there was a decreasing gradient of labeling from the edge of necrosis. Some labeling was also found in various cell types of young, healthy stems and was shown to accumulate in large amounts in the same cell types after the deposition of an elicitor solution at the top of the decapitated plant.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:36:20