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Titolo:
CLONING, EXPRESSION AND CLASSIFICATION OF A KAPPA(3)-RELATED OPIOID RECEPTOR USING ANTISENSE OLIGODEOXYNUCLEOTIDES
Autore:
PAN YX; CHENG J; XU J; PASTERNAK GW;
Indirizzi:
CORNELL UNIV,COLL MED,MEM SLOAN KETTERING CANC CTR,COTZIAS LAB NEUROONCOL NEW YORK NY 10021 CORNELL UNIV,COLL MED,DEPT NEUROL & NEUROSCI NEW YORK NY 10021 CORNELL UNIV,COLL MED,DEPT PHARMACOL NEW YORK NY 10021
Titolo Testata:
Regulatory peptides
fascicolo: 1, volume: 54, anno: 1994,
pagine: 217 - 218
SICI:
0167-0115(1994)54:1<217:CEACOA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NALOXONE BENZOYLHYDRAZONE; OPIATE; KAPPA; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
Y.X. Pan et al., "CLONING, EXPRESSION AND CLASSIFICATION OF A KAPPA(3)-RELATED OPIOID RECEPTOR USING ANTISENSE OLIGODEOXYNUCLEOTIDES", Regulatory peptides, 54(1), 1994, pp. 217-218

Abstract

Using degenerate oligodeoxynucleotide primers based upon the predicted amino acid sequence of the cloned delta receptor in RT-PCR we isolated a cDNA of approximately 500 bases with high homology to the cloned delta, mu and kappa, receptors. Before proceeding to clone the full length coding region, we designed an antisense oligodeoxynucleotide (ODN) and examined ifs actions in vivo. Administered intracerebroventricularly, the antisense ODN effectively blocks the analgesic actions of the kappa, agent naloxone benzoylhydrazone (NalBzoH) without affecting morphine (mu) analgesia. A mismatch ODN identical in base composition to the antisense but with four bases out of sequence is inactive. Having established the relevance of the sequence, we proceeded to clone thefull length cDNA from a mouse brain library and characterize the expressed protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 00:17:19