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Titolo:
PHARMACOLOGICAL ENHANCEMENT OF IN-VIVO FOREIGN GENE-EXPRESSION IN MUSCLE
Autore:
DANKO I; FRITZ JD; JIAO SS; HOGAN K; LATENDRESSE JS; WOLFF JA;
Indirizzi:
UNIV WISCONSIN,WAISMAN CTR,DEPT PEDIAT,ROOM 355,1500 HIGHLAND AVE MADISON WI 53705 UNIV WISCONSIN,WAISMAN CTR,DEPT PEDIAT MADISON WI 53705 UNIV WISCONSIN,WAISMAN CTR,DEPT MED GENET MADISON WI 53705 UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,DEPT ANESTHESIOL MADISON WI 53706 UNIV WISCONSIN,CTR CLIN SCI MADISON WI 53706
Titolo Testata:
Gene therapy
fascicolo: 2, volume: 1, anno: 1994,
pagine: 114 - 121
SICI:
0969-7128(1994)1:2<114:PEOIFG>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE SKELETAL-MUSCLE; MUSCULAR-DYSTROPHY; LOCAL-ANESTHETICS; MAMMALIAN-CELLS; PLASMID DNA; LONG-TERM; REGENERATION; INVIVO; RAT; BUPIVACAINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
I. Danko et al., "PHARMACOLOGICAL ENHANCEMENT OF IN-VIVO FOREIGN GENE-EXPRESSION IN MUSCLE", Gene therapy, 1(2), 1994, pp. 114-121

Abstract

Intramuscular injection of naked plasmid DNA provides a means for gene transfer and expression in striated muscle. in this study, the effects oi treating muscle with normal saline, etidocaine, mepivacaine, acetic anhydride, sodium bicarbonate, Notechis scutatus venom, cardiotoxin and bupivacaine before plasmid DNA injection on foreign gene expression were evaluated Dose dependence, strain and species specificity, the time interval between pharmacological agent and plasmid DNA injection, the stability of gene expression and the Tate of the injected plasmid DNA were studied using reporter gene expression, by histological examination and semi-quantitative polymerase chain reaction. Of the various gents tested, the best enhancement of foreign gene expression occurred in muscle treated with 0.75% bupivacaine five to seven days before plasmid DNA injection. Rat and mouse quadriceps muscle treated with 0.75% bupivacaine had levels of luciferase activity four- to 40-times greater than non-bupivacaine-treated muscle. Also, beta-galactosidase expressing myofibers were observed throughout the length of the musclein samples treated with 0.75% bupivacaine before reporter gene injection. Muscle treated with 0.75% bupivacaine fully recovered from the degeneration caused by its injection with no long-term effects histologically. The heightened level of reporter gene expression persisted in 0.75% bupivacaine-treated muscle for one month, but decreased to that of non-bupivacaine-treated muscle by two months after plasmid DNA injection. Enhancement oi foreign gene expression may be particularly advantageous in vaccination protocols employing intramuscular plasmid injection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 17:30:21