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Titolo:
RESISTANCE OF ENTEROCOCCUS-FAECIUM TO NEUTROPHIL-MEDIATED PHAGOCYTOSIS
Autore:
ARDUINO RC; JACQUESPALAZ K; MURRAY BE; RAKITA RM;
Indirizzi:
UNIV TEXAS,SCH MED,CTR INFECT DIS,DEPT INTERNAL MED,6431 FANNIN,1728 JFB HOUSTON TX 77030 UNIV TEXAS,SCH MED,CTR INFECT DIS,DEPT INTERNAL MED HOUSTON TX 77030 UNIV TEXAS,SCH MED,DEPT MICROBIOL & MOLEC GENET HOUSTON TX 77030
Titolo Testata:
Infection and immunity
fascicolo: 12, volume: 62, anno: 1994,
pagine: 5587 - 5594
SICI:
0019-9567(1994)62:12<5587:ROETNP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALTERNATIVE COMPLEMENT PATHWAY; FIELD GEL-ELECTROPHORESIS; GROUP-B STREPTOCOCCI; BETA-LACTAMASE; GENTAMICIN-RESISTANT; SIALIC-ACID; FAECALIS; MECHANISMS; INFECTIONS; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
R.C. Arduino et al., "RESISTANCE OF ENTEROCOCCUS-FAECIUM TO NEUTROPHIL-MEDIATED PHAGOCYTOSIS", Infection and immunity, 62(12), 1994, pp. 5587-5594

Abstract

During a previous study of the opsonic requirements for neutrophil (polymorphonuclear leukocyte [PMN])-mediated killing of enterococci, we identified two strains of Enterococcus faecium (TX0015 and TX0016) that were resistant to PMN-mediated killing. To better define the mechanism of this resistance, we examined phagocytosis with a fluorescence assay and found that TX0016 was completely resistant to phagocytosis by PMNs; this finding was confirmed by electron microscopy. Examination of multiple strains of enterococci revealed that all 20 strains of Enterococcus faecalis tested were readily phagocytosed (mean, 18 intracellular organisms per PMN; range, 7 to 28). In contrast, only 13 (50%) of26 strains of E. faecium tested were susceptible to phagocytosis (greater than or equal to 7 organisms per PMN); the other 13 strains showed less than or equal to 3 organisms per PMN. Enterococcus casseliflavus ATCC 25788 and one strain of Enterococcus hirae were also resistant to phagocytosis, while two strains of Enterococcus durans, Enterococcus mundtii ATCC 43186, and one strain each of Enterococcus raffinosus and Enterococcus solitarius were readily phagocytosed. Exposure of E. faecium TX0016 to sodium periodate, but not to the protease trypsin or pronase or to phospholipase C, eliminated resistance to phagocytosis. Sialic acid, a common periodate-sensitive structure used by microorganisms to resist opsonization, could not be demonstrated in E. faecium TX0016 by the thiobarbituric acid method, nor was phagocytosis of TX0016 altered by neuraminidase treatment, This study suggests that there is a difference in susceptibility to phagocytosis by PMNs between different species of enterococci and that a carbohydrate-containing moiety which is not sialic acid may be involved in the resistance of E. faecium TX0016 to phagocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 08:19:49