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Titolo:
INCREASE OF GAMMA DELTA T-CELLS IN-HOSPITAL WORKERS WHO ARE IN CLOSE CONTACT WITH TUBERCULOSIS PATIENTS/
Autore:
UETA C; TSUYUGUCHI I; KAWASUMI H; TAKASHIMA T; TOBA H; KISHIMOTO S;
Indirizzi:
OSAKA PREFECTURAL HABIKINO HOSP,3-7-1 HABIKINO HABIKINO OSAKA 583 JAPAN
Titolo Testata:
Infection and immunity
fascicolo: 12, volume: 62, anno: 1994,
pagine: 5434 - 5441
SICI:
0019-9567(1994)62:12<5434:IOGDTI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-GAMMA-DELTA; MYCOBACTERIUM-TUBERCULOSIS; PERIPHERAL-BLOOD; LYMPHOCYTES-T; EXOGENOUS REINFECTION; IMMUNE-RESPONSE; ENTEROTOXIN-B; ANTIGEN; MICE; RECOGNITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
C. Ueta et al., "INCREASE OF GAMMA DELTA T-CELLS IN-HOSPITAL WORKERS WHO ARE IN CLOSE CONTACT WITH TUBERCULOSIS PATIENTS/", Infection and immunity, 62(12), 1994, pp. 5434-5441

Abstract

gamma/delta T cells are likely to participate in the immune response to tuberculous infection in humans. In this study, we carried out an investigation to characterize the responsiveness of gamma/delta T cellsfrom tuberculous patients and healthy individuals to mycobacterial stimulation in vitro. Healthy subjects were assigned to the following two groups: those who had been exposed to tuberculosis (contacts) and those who had not been exposed (noncontacts). The percent gamma/delta T tells in fresh peripheral blood obtained from health care workers who were tuberculin skin test positive and who had constant contact with patients with active tuberculosis (healthy contacts) was significantly higher, whereas healthy noncontacts showed the normal range of gamma/delta T cells. Patients with active pulmonary tuberculosis also had lowlevels of gamma/delta T cells. HLA-DR antigen-bearing activated gamma/delta T cells were observed in higher percentages among healthy contacts than among healthy noncontacts or patients with pulmonary tuberculosis. In healthy contacts, gamma/delta T cells increased as a percentage of peripheral blood mononuclear cells after in vitro stimulation with purified protein derivative (PPD) tuberculin compared with the percentage of fresh peripheral blood mononuclear cells that they made up, whereas no such increase was observed in patients with tuberculosis orin healthy noncontact. Phenotypic analysis of the gamma/delta T cellsin healthy contacts, which increased in number in vitro in response to PPD, revealed the preferential outgrowth of CD4(+) V gamma 2(+) gamma/delta T cells. This expansion of gamma/delta T cells by PPD requiredaccessory cells, and it was inhibited by the addition of an antibody against HLA-DR in culture. Proteolytic digestion of PPD showed that gamma/delta T cells increased in number in response to peptide, but not nonpeptide, components of PPD. These findings suggest that gamma/deltaT tells, especially CD4(+) V gamma 2(+) gamma/delta T cells, may participate in the immune surveillance of tuberculous infections in humans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:20:37