Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF THE EXTRACELLULAR GALACTOMANNAN OF ASPERGILLUS-FUMIGATUS
Autore:
LATGE JP; KOBAYASHI H; DEBEAUPUIS JP; DIAQUIN M; SARFATI J; WIERUSZESKI JM; PARRA E; BOUCHARA JP; FOURNET B;
Indirizzi:
INST PASTEUR,UNITE MYCOL,25 RUE DR ROUX F-75724 PARIS 15 FRANCE UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB F-59655 VILLENEUVE DASCQ FRANCE CTR HOSP REG,PARASITOL MYCOL LAB F-49100 ANGERS FRANCE CSIC,INST QUIM ORGAN,CARBOHIDRATOS GRP E-28006 MADRID SPAIN
Titolo Testata:
Infection and immunity
fascicolo: 12, volume: 62, anno: 1994,
pagine: 5424 - 5433
SICI:
0019-9567(1994)62:12<5424:CAICOT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR MAGNETIC-RESONANCE; GAS-LIQUID-CHROMATOGRAPHY; 5-O-BETA-D-GALACTOFURANOSYL-CONTAINING EXOCELLULAR GLYCOPEPTIDE; PENICILLIUM-CHARLESII; METHYL GLYCOSIDES; CELL-WALL; MANNOPYRANOSYL RESIDUES; INVASIVE ASPERGILLOSIS; GLYCOPROTEIN GLYCANS; STRUCTURAL-ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
J.P. Latge et al., "CHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF THE EXTRACELLULAR GALACTOMANNAN OF ASPERGILLUS-FUMIGATUS", Infection and immunity, 62(12), 1994, pp. 5424-5433

Abstract

The galactomannan (GM) produced extracellularly by Aspergillus fumigatus has been purified by a double sequential hydrazine-nitrous acid treatment of the ethanol precipitate of the culture filtrate. Nuclear magnetic resonance and gas-liquid chromatography-mass spectrometry analysis have been performed on intact GM, acid-hydrolyzed GM, and oligomers resulting from the acetolysis of the acid-hydrolyzed GM. Results show that A. fumigatus GM is composed of a linear mannan core with an alpha-(1-2)-linked mannotetraose repeating unit attached via alpha-(1-6) linkage. Side chains composed of an average of 4 to 5 beta-(1-5)-galactofuranose units are linked to C-6 and C-3 positions of alpha-(1-2)-linked mannose units of the mannan. The immunoreactivity of GM and HCl-hydrolyzed GM was studied by use of human sera from aspergillosis patients and an antigalactofuran monoclonal antibody. The alpha-(1-2) (1-6)-mannan core is not antigenic The immunogenic galactofuran is found amongst several exocellular glycoproteins. According to a direct enzyme-linked immunosorbent assay with GM as the detector antigen, only 26% of the serum samples from aspergilloma patients (all positive by immunodiffusion assays) give optical density values superior to a cutoff estimated as the mean +/- 3 standard deviations of values obtained with control sera.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:50:14