Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CONFORMATIONAL-CHANGES IN OXIDIZED LDL RECOGNIZED BY MOUSE PERITONEAL-MACROPHAGES
Autore:
MAEBA R; SHIMASAKI H; UETA N;
Indirizzi:
TEIKYO UNIV,SCH MED,DEPT BIOCHEM,ITABASHI KU,2-11-1 KAGA TOKYO 173 JAPAN
Titolo Testata:
Biochimica et biophysica acta, L. Lipids and lipid metabolism
fascicolo: 1-2, volume: 1215, anno: 1994,
pagine: 79 - 86
SICI:
0005-2760(1994)1215:1-2<79:CIOLRB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN MONOCYTE-MACROPHAGES; LIPID-PEROXIDATION; FAMILIAL HYPERCHOLESTEROLEMIA; CHOLESTEROL DEPOSITION; OXIDATIVE MODIFICATION; SCAVENGER RECEPTOR; PRODUCTS; ATHEROSCLEROSIS; ACCUMULATION;
Keywords:
MACROPHAGE; OXIDIZED LDL; AGGREGATION; FLUORESCENT PRODUCT; SCHIFF BASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
R. Maeba et al., "CONFORMATIONAL-CHANGES IN OXIDIZED LDL RECOGNIZED BY MOUSE PERITONEAL-MACROPHAGES", Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1215(1-2), 1994, pp. 79-86

Abstract

Mouse peritoneal macrophages have been considered to recognize and take up oxidized LDL by a scavenger receptor. However, it is still unknown what conformational changes in oxidized LDL contribute to recognition by the macrophage scavenger receptor. In the present study, it was shown that the amount of oxidized LDL taken up by macrophages correlated well with the fluorescence intensity formed in oxidized LDL. The autofluorescent products generated in oxidized LDL were characterized byEx:365 nm Em:430 nm, and the intensity of the fluorescence was reduced at base pH, and restored by adjusting the pH to neutral. The characteristics of the fluorescent products indicate that a Schiff base structure was formed in oxidized LDL. Oxidized LDLs were fractionated into native size and aggregated large particles with HPLC by monitoring fluorescence, It was demonstrated that macrophages ingest selectively or preferentially aggregated oxidized LDL, but not native size oxidized LDL. The incorporation of aggregated oxidized LDL was remarkably suppressed by heparin and cytochalasin B. These results suggest that mouse peritoneal macrophages recognize the conformational changes in oxidizedLDL related to the formation of a Schiff base structure with increasing autofluorescence, and ingest selectively aggregated large particlesin oxidized LDL in a phagocytic process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 03:21:07