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Titolo:
ROLE OF CATIONIC PROTEINS IN THE AIRWAY - HYPERRESPONSIVENESS DUE TO AIRWAY INFLAMMATION
Autore:
COYLE AJ; UCHIDA D; ACKERMAN SJ; MITZNER W; IRVIN CG;
Indirizzi:
NATL JEWISH CTR IMMUNOL & RESP MED,PULM PHYSIOL UNIT,1400 JACKSON ST DENVER CO 80206 NATL JEWISH CTR IMMUNOL & RESP MED,DEPT PEDIAT,DIV PULM SCI DENVER CO80206 NATL JEWISH CTR IMMUNOL & RESP MED,DEPT MED DENVER CO 80206 BETH ISRAEL HOSP,DEPT MED,DIV INFECT DIS BOSTON MA 02215 HARVARD UNIV,SCH MED BOSTON MA 02115 JOHNS HOPKINS UNIV,DEPT ENVIRONM HLTH SCI,DIV ENVIRONM PHYSIOL BALTIMORE MD 00000
Titolo Testata:
American journal of respiratory and critical care medicine
fascicolo: 5, volume: 150, anno: 1994, supplemento:, S
pagine: 190000063 - 190000071
SICI:
1073-449X(1994)150:5<190000063:ROCPIT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR BASIC-PROTEIN; HUMAN EOSINOPHIL GRANULE; LATE ASTHMATIC REACTIONS; ANTIGEN-INDUCED AIRWAY; BRONCHIAL-ASTHMA; SMOOTH-MUSCLE; BRONCHOALVEOLAR LAVAGE; TRACHEAL EPITHELIUM; GUINEA-PIGS; ALLERGEN CHALLENGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
93
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Coyle et al., "ROLE OF CATIONIC PROTEINS IN THE AIRWAY - HYPERRESPONSIVENESS DUE TO AIRWAY INFLAMMATION", American journal of respiratory and critical care medicine, 150(5), 1994, pp. 190000063-190000071

Abstract

Major basic protein (MBP) is a highly cationic protein found in the granules of eosinophils. It has been postulated that MBP may participate in the pathogenesis of airway hyperresponsiveness exhibited by asthmatic patients. Accordingly, we have employed a rat system to investigate the effect of human MBP instillation on airway responsiveness and the possible role of cationic charge in the determination of this effect. Major basic protein caused a significant increase in airway responsiveness to inhaled methacholine. Two polycations, poly-L-arginine and poly-L-lysine, also increased airway responsiveness to inhaled methacholine. Moreover, two other very different cationic proteins, platelet factor 4 (PF4) and cathepsin G were also capable of inducing airway hyperresponsiveness. These effects were dependent on their positive charge, since the charge - and, hence the effect - of these proteins was neutralized with low molecular weight heparin. In addition, other polyanions, such as low molecular weight heparin, albumin, or dextran sulfate, were also effective. We investigated whether two synthetic cationic proteins, poly-L-arginine and poly-L-lysine, could modify epithelial-dependent responses using a perfused guinea pig tracheal tube preparation. With an intact epithelium, methacholine was some 150 times less potent when applied intraluminally than when applied extraluminally. Perfusion of the luminal surface with cationic proteins increased the potency of intraluminally applied methacholine without modifying the responses to extraluminally applied methacholine. Cationic proteins alsoattenuated the relaxant effects of intraluminally applied KCl. These effects occurred in the absence of any overt epithelial cell damage. Our data demonstrates that cationic proteins can modify epithelial-dependent responses in the airways. While the precise mechanisms are unclear, a role is suggested for a charge-mediated interaction with the respiratory epithelium, resulting in airway smooth muscle dysfunction. Lastly, we suggest that it is the total cationic load formed by all of the inflammatory cells that may have importance in the pathogenesis of asthma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 22:38:03