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Titolo:
NEURAL REGULATION OF ACETYLCHOLINESTERASE MESSENGER-RNAS AT MAMMALIANNEUROMUSCULAR SYNAPSES
Autore:
MICHEL RN; VU CQ; TETZLAFF W; JASMIN BJ;
Indirizzi:
UNIV OTTAWA,FAC MED,DEPT PHYSIOL,451 SMYTH RD OTTAWA K1H 8M5 ON CANADA UNIV OTTAWA,FAC MED,DEPT PHYSIOL OTTAWA K1H 8M5 ON CANADA
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 127, anno: 1994,
pagine: 1061 - 1069
SICI:
0021-9525(1994)127:4<1061:NROAMA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREFERENTIAL SYNAPTIC EXPRESSION; CHARACTERIZING FAST MUSCLES; RECEPTOR MESSENGER-RNA; RAT SOLEUS MUSCLE; MOLECULAR-FORMS; SKELETAL-MUSCLE; GENE-EXPRESSION; NEUROTROPHIC REGULATION; BOTULINUM TOXIN; SINGLE GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
73
Recensione:
Indirizzi per estratti:
Citazione:
R.N. Michel et al., "NEURAL REGULATION OF ACETYLCHOLINESTERASE MESSENGER-RNAS AT MAMMALIANNEUROMUSCULAR SYNAPSES", The Journal of cell biology, 127(4), 1994, pp. 1061-1069

Abstract

We examined the role of innervation on acetylcholinesterase (AChE) gene expression within mammalian skeletal muscle fibers. First, we showed the selective accumulation of AChE mRNAs within the junctional vs extrajunctional sarcoplasm of adult muscle fibers using a quantitative reverse transcription PCR assay and demonstrated by in situ hybridization experiments that AChE transcripts are concentrated immediately beneath the postsynaptic membrane of the neuromuscular junction. Next, we determined the influence of nerve-evoked activity vs putative trophic factors on the synaptic accumulation of AChE mRNA levels in muscle fibers paralyzed by either surgical denervation or selective blockage of nerve action potentials with chronic superfusion of tetrodotoxin. Our results indicated that muscle paralysis leads to, a marked decrease inAChE transcripts from the postsynaptic sarcoplasm, yet the extent of this decrease is less pronounced after tetrodotoxin inactivation than after denervation. These results suggest that although nerve-evoked activity per se appears a key regulator of AChE mRNA levels, the integrity of the synaptic structure or the release of putative trophic factors contribute to maintaining the synaptic accumulation of AChE transcripts at adult neuromuscular synapses. Furthermore, the pronounced downregulation of AChE transcripts in paralyzed muscles stands in sharp contrast to the well-documented increase in nicotinic acetylcholine receptor mRNAs under these conditions, and indicates that expression of thegenes encoding these two synaptic proteins are subjected to differentregulatory mechanisms in adult muscle fibers in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:58:56