Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ALVEOLAR EPITHELIAL COMPOSITION AND ARCHITECTURE OF THE LATE FETAL PULMONARY ACINUS - AN IMMUNOCYTOCHEMICAL AND MORPHOMETRIC STUDY IN A RATMODEL OF PULMONARY HYPOPLASIA AND CONGENITAL DIAPHRAGMATIC-HERNIA
Autore:
BRANDSMA AE; TENHAVEOPBROEK AAW; VULTO IM; MOLENAAR JC; TIBBOEL D;
Indirizzi:
DEPT ANAT & EMBRYOL,RESP BIOL GRP,POB 9602 2300 RC LEIDEN NETHERLANDS LEIDEN UNIV,DEPT PULM LEIDEN NETHERLANDS LEIDEN UNIV,DEPT PEDIAT SURG LEIDEN NETHERLANDS UNIV ROTTERDAM,DEPT PULM ROTTERDAM NETHERLANDS UNIV ROTTERDAM,DEPT PEDIAT SURG ROTTERDAM NETHERLANDS
Titolo Testata:
Experimental lung research
fascicolo: 6, volume: 20, anno: 1994,
pagine: 491 - 515
SICI:
0190-2148(1994)20:6<491:AECAAO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUNG DEVELOPMENT; MOUSE EMBRYO; II CELLS; TYPE-2 CELLS; MORPHOLOGY; REPAIR; CYTOCHEMISTRY; MATURATION; APPEARANCE; MICROSCOPY;
Keywords:
PULMONARY HYPOPLASIA; TYPE II ALVEOLAR EPITHELIAL CELLS; TYPE I ALVEOLAR EPITHELIAL CELLS; CONGENITAL DIAPHRAGMATIC HERNIA; NITROFEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
A.E. Brandsma et al., "ALVEOLAR EPITHELIAL COMPOSITION AND ARCHITECTURE OF THE LATE FETAL PULMONARY ACINUS - AN IMMUNOCYTOCHEMICAL AND MORPHOMETRIC STUDY IN A RATMODEL OF PULMONARY HYPOPLASIA AND CONGENITAL DIAPHRAGMATIC-HERNIA", Experimental lung research, 20(6), 1994, pp. 491-515

Abstract

The aim of the present study was to compare the architecture and alveolar epithelial cell composition of the pulmonary acinus in hypoplastic and normal fetal rat lungs. For this purpose, a rat model of pulmonary hypoplasia in association with congenital diaphragmatic hernia (CDH) induced by Nitrofen (100 mg on day 10 of pregnancy) was studied. Sections (5 mu m) from lungs of control and Nitrofen-exposed fetal Sprague Dawley rats with or without CDH aged 18-22 days (vaginal plug on day1, birth on day 23) were stained with hematoxylin and eosin. To identify developing alveolar epithelial cells, sections were incubated withanti-surfactant protein A (SP-A; rabbit anti-mouse) or preimmunization serum (indirect immunofluorescence). On days 18 and 19, control lungs and exposed lungs from fetuses with and without CDH looked similar (pseudoglandular stage of lung development). The prospective pulmonary acinus consisted of acinar tubules with small round lumens, lined by cuboid, fluorescent type II cells. Morphometric analysis on day 19 showed significantly smaller lung volumes and lung tissue volumes after Nitrofen exposure. On day 20 (canalicular stage), some tubules were slightly dilated and lined by cuboid and thinner fluorescent cells; these dilated tubules were less numerous in lungs from exposed fetuses with CDH. On days 21 and 22 (saccular stage), the saccular lining consistedof cuboid to thin fluorescent cells in exposed lungs from fetuses with and without CDH, and fluorescent (low) cuboid cells interspersed with dark zones (type I cell areas) in control lungs. In the exposed lungs from fetuses with CDH, the lumens of all airspaces were frequently slit-like, and the septa were thicker. These phenomena gave the lungs aprimitive, compact aspect. Morphometric analysis on day 22 showed smaller lung volumes and lung tissue volumes, smaller airspace/tissue ratios, smaller epithelial surface areas, and more type II cells per surface area in Nitrofen-exposed lungs than in normal control lungs. The results suggest that Nitrofen-exposed, and thus hypoplastic, fetal rat lungs are retarded with respect to the differentiation of cuboid type II cells into squamous type I cells whether or not CDH is present, andwith respect to the development of the future airspaces between days 20 and 22 if CDH is present.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:03:02