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Titolo:
2 FORMS OF THE MB-1 GENE TRANSCRIPT IN CATTLE
Autore:
YOUN HY; GOITSUKA R; OKUDA M; WATARI T; TSUJIMOTO H; HASEGAWA A;
Indirizzi:
UNIV TOKYO,FAC AGR,DEPT VET INTERNAL MED,BUNKYO KU,1-1-1 YAYOI TOKYO 113 JAPAN
Titolo Testata:
The Journal of immunology
fascicolo: 11, volume: 153, anno: 1994,
pagine: 5127 - 5132
SICI:
0022-1767(1994)153:11<5127:2FOTMG>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL ANTIGEN-RECEPTOR; MOLECULAR-CLONING; B-CELLS; BOVINE LEUKEMIA; IG-BETA; COMPLEX; EXPRESSION; SUBUNIT; ALPHA; B29;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
H.Y. Youn et al., "2 FORMS OF THE MB-1 GENE TRANSCRIPT IN CATTLE", The Journal of immunology, 153(11), 1994, pp. 5127-5132

Abstract

We have isolated two forms of bovine mb-1 cDNA clones from a cDNA library of a bovine leukemia virus-infected B-lymphoblastoid cell line. The nucleotide sequence analysis of these cDNA clones indicated that the shorter form of cDNA had a 119-bp deletion that precisely corresponded to the exon III domain of the human and murine mb-1 genes. This deletion would result in a frame shift, producing a premature translationtermination leading to the lack of the entire transmembrane and cytoplasmic domains of the normal mb-1 molecule. The existence of two species of mRNA transcripts corresponding to the isolated cDNA clones were confirmed in all of the bovine leukemia cell lines examined by PCR with the use of the primers flanking the deletion site. Furthermore, the shorter, mb-1 transcript was induced in normal PBMC by stimulation with mitogens or culture supernatants of bovine leukemia virus-infected cell lines. These findings indicate the presence of the truncated mb-1 gene product which may have some function different from that of the normal mb-1 gene product.

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Documento generato il 27/11/20 alle ore 21:45:33