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Titolo:
TGF-BETA-1 IS A POTENT INDUCER OF HUMAN EFFECTOR T-CELLS
Autore:
CERWENKA A; BEVEC D; MAJDIC O; KNAPP W; HOLTER W;
Indirizzi:
UNIV VIENNA,SFI,VIRCC,INST IMMUNOL,BORSCHKEGASSE 8A A-1090 VIENNA AUSTRIA UNIV VIENNA,SFI,VIRCC,INST IMMUNOL A-1090 VIENNA AUSTRIA ST ANNA CHILDRENS HOSP,CHILDRENS CANC RES INST A-1090 VIENNA AUSTRIA SANDOZ GMBH,RES INST VIENNA AUSTRIA
Titolo Testata:
The Journal of immunology
fascicolo: 10, volume: 153, anno: 1994,
pagine: 4367 - 4377
SICI:
0022-1767(1994)153:10<4367:TIAPIO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMING GROWTH-FACTOR; INTESTINAL INTRAEPITHELIAL LYMPHOCYTES; FC-GAMMA-RIII; FACTOR-BETA; MONOCLONAL-ANTIBODY; IGA PRODUCTION; KILLER CELLS; HELPER-CELLS; INHIBITION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
A. Cerwenka et al., "TGF-BETA-1 IS A POTENT INDUCER OF HUMAN EFFECTOR T-CELLS", The Journal of immunology, 153(10), 1994, pp. 4367-4377

Abstract

TGF-beta 1 is known to modulate lymphocyte activation affecting cell proliferation and the production of cytokines and Igs. Little is knownabout the characteristics of T cells grown in the presence of TGF-beta 1. We have stimulated human T cells with PHA in the presence of TGF-beta 1 under serum-free conditions for 7 days and characterized the resulting cell population. TGF-beta 1 (0.0032 to 10 ng/ml) affected neither [H-3]thymidine incorporation (day 4) nor cell yield (day 7) in these cultures. However, cells activated in the presence of TGF-beta 1 proliferated vigorously in secondary cultures and produced highly elevated amounts of IL-2 (12 +/- 3 -fold enhancement of IL-2 production in response to CD2 plus CD28 stimulation compared with control cells, mean+/- SEM; n = 10). The enhancing effects of TGF-beta 1 were demonstrable over a wide range of concentrations (0.4 to 10 ng/ml). The increased IL-2 protein production was paralleled by a dramatic up-regulation of IL-2 mRNA. In addition, cells precultured with TGF-beta 1 responded with enhanced cluster formation in the secondary cultures. With regardto their phenotype, we observed an increased expression of the alpha(E) beta(7)-integrin human mucosal lymphocyte-1 and of the CD2-restricted epitope CD2R, whereas the expression of CD11a was slightly decreased. In contrast, TGF-beta 1 did not influence the constitutive or activation-induced expression of CD4, CD8, CD45RA, CD45RO, CD25, CD71, CD54, CD58, CD59, and B7. We conclude that TGF-beta 1 supports the generation of human effector cells with a strongly enhanced capacity to respond to subsequent restimulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 04:10:39