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Titolo:
SOLUBILITY AND PRENUCLEATION OF APROTININ (BPTI) MOLECULES IN SODIUM-CHLORIDE SOLUTIONS
Autore:
LAFONT S; VEESLER S; ASTIER JP; BOISTELLE R;
Indirizzi:
CTR RECH MECAN CROISSANCE CRISTALLINE,CNRS,CAMPUS LUMINY,CASE 913 F-13288 MARSEILLE 09 FRANCE CTR RECH MECAN CROISSANCE CRISTALLINE,CNRS F-13288 MARSEILLE 09 FRANCE
Titolo Testata:
Journal of crystal growth
fascicolo: 3-4, volume: 143, anno: 1994,
pagine: 249 - 255
SICI:
0022-0248(1994)143:3-4<249:SAPOA(>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC TRYPSIN-INHIBITOR; EXPONENTIAL-SAMPLING METHOD; DILATIONALLY INVARIANT TRANSFORMS; CONCENTRATION-DEPENDENCE; CORRELATION SPECTROSCOPY; DIFFUSION-COEFFICIENT; LIGHT-SCATTERING; POLYDISPERSITY; PROTEIN; LAPLACE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
S. Lafont et al., "SOLUBILITY AND PRENUCLEATION OF APROTININ (BPTI) MOLECULES IN SODIUM-CHLORIDE SOLUTIONS", Journal of crystal growth, 143(3-4), 1994, pp. 249-255

Abstract

In the present study we describe the first stage of the crystallization process of the bovine pancreatic trypsin inhibitor BPTI from sodiumchloride solutions, with special attention to the polydispersity of the particle size distribution. First, we measured the solubility of BPTI at four NaCl concentrations (1.4 to 2.3 mol/l) and four temperatures (5 to 25 degrees C). The solubility of the protein decreases with increasing temperature and increasing ionic strength, the effect of temperature being more pronounced at low ionic strength. Second, we investigated the behaviour of the BPTI molecules by quasi-elastic light scattering (QELS) measurements at different concentrations in protein (15 to 110 mg/ml), and ionic strengthes (0 to 2.3 mol/l NaCl). The solutions become monodisperse, but not monomeric, in the vicinity of the solubility curve. They are stable until nucleation takes place but it is impossible to decide whether these aggregates are the growth units of the crystals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 05:34:38