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Titolo:
CELL-DENSITY CAN AFFECT CHOLESTERYL ESTER ACCUMULATION IN THE HUMAN THP-I MACROPHAGE
Autore:
RODRIGUEZ A; KAFONEK SD; GEORGOPOULOS A; BACHORIK PS;
Indirizzi:
SINAI HOSP,DEPT MED,DIV ENDOCRINOL & METAB,2401 W BELVEDERE AVE BALTIMORE MD 21215 JOHNS HOPKINS UNIV,SCH MED,DEPT MED BALTIMORE MD 21287 JOHNS HOPKINS UNIV,SCH MED,DEPT PEDIAT & LAB MED BALTIMORE MD 21287
Titolo Testata:
Journal of lipid research
fascicolo: 11, volume: 35, anno: 1994,
pagine: 1909 - 1917
SICI:
0022-2275(1994)35:11<1909:CCACEA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONOCYTE-MACROPHAGES; FOAM CELLS; LIPOPROTEIN RECEPTOR; LINE THP-1; SCAVENGER RECEPTOR; INDUCTION; PLASMA;
Keywords:
FOAM CELLS; ATHEROSCLEROSIS; MODIFIED LDL; CELLULAR CHOLESTEROL METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
A. Rodriguez et al., "CELL-DENSITY CAN AFFECT CHOLESTERYL ESTER ACCUMULATION IN THE HUMAN THP-I MACROPHAGE", Journal of lipid research, 35(11), 1994, pp. 1909-1917

Abstract

Esterified cholesterol (EC) accumulation was induced in THP-1 macrophages after exposure to acetylated LDL (acLDL), and the extent of accumulation was dependent on cell density. EC mass was 5-fold greater in cells plated at 1.0 x 10(6) cells/35 mm dish compared to cells plated at density 4.0 x 10(6) cells/dish. In addition, [C-14]oleate incorporation into EC also increased with decreasing cell number, with 4-fold greater incorporation (6 h: 177 +/- 0.014 vs. 45 +/- 0.001 pmol/mg cell protein, P < 0.001; 24 h: 515 +/- 0.037 vs. 120 +/- 0.012 pmol/mg, P <0.001) in cells plated less densely compared to cells plated at a higher density. The rate of I-125-labeled acLDL degradation was about P-fold greater in cells plated at the lower density (105 vs. 60 ng/h per mg cell protein). Northern analysis showed a 2-fold reduction in the expression of human scavenger receptor mRNA in densely plated cells, and immunoprecipitation also demonstrated a 2-fold decrease in scavengerreceptor protein. Conditioned media did not differentially affect EC formation at either cell density. Fatty acid supplementation increasedEC formation and the proportion of esterified sterol content only in cells plated at the higher density The fatty acid effect was also seenwhen cells were exposed to P-VLDL, which induced comparable levels ofEC accumulation by non-scavenger receptor-mediated processes in densely plated cells. Foam cell formation in THP-1 macrophages may depend on cell density, which appears to affect both scavenger and non-scavenger receptor activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 03:39:56