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Titolo:
DISCRIMINATION OF MYCOBACTERIUM-AVIUM MYCOBACTERIUM-INTRACELLULARE STRAINS BY GENOMIC DNA-FINGERPRINTING WITH A 16S RIBOSOMAL-RNA GENE PROBE
Autore:
PEILLON R; DROUET EB; BRUNEAU S; PANTEIX G; DENOYEL GA; DEMONTCLOS HP;
Indirizzi:
INST PASTEUR,UNITE INFECTOL,AVE TONY GARNIER F-69365 LYON 7 FRANCE INST PASTEUR,UNITE INFECTOL F-69365 LYON 7 FRANCE
Titolo Testata:
FEMS microbiology letters
fascicolo: 1, volume: 124, anno: 1994,
pagine: 75 - 79
SICI:
0378-1097(1994)124:1<75:DOMMS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENES; COMPLEX; AIDS; GENERATION; DIVERSITY;
Keywords:
MYCOBACTERIUM AVIUM MYCOBACTERIUM INTRACELLULARE; DNA FINGERPRINTING; NONRADIOACTIVE PROBE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
R. Peillon et al., "DISCRIMINATION OF MYCOBACTERIUM-AVIUM MYCOBACTERIUM-INTRACELLULARE STRAINS BY GENOMIC DNA-FINGERPRINTING WITH A 16S RIBOSOMAL-RNA GENE PROBE", FEMS microbiology letters, 124(1), 1994, pp. 75-79

Abstract

Ribotyping was investigated as a means of distinguishing ten different serotyped reference strains and seven epidemiologically unrelated isolates of Mycobacterium avium-Mycobacterium intracellulare using a labelled 16S rDNA probe. Thirteen restriction enzymes were screened towards an accurate discrimination of strains. Two selected restriction enzymes (SacI and ClaI) enabled us to classify the 17 strains into ten ribotypes with an index of discrimination of 0.897. Typeability and reproducibility of the method reached 100%. The patterns obtained exhibited polymorphism of RE fragments within and outside the 16S rRNA gene and may be useful for epidemiological studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:22:10