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Titolo:
TRANSPOSON-INDUCED PIGMENT-DEFICIENT MUTANTS OF PORPHYROMONAS-GINGIVALIS
Autore:
HOOVER CI; YOSHIMURA F;
Indirizzi:
AICHI GAKUIN UNIV,SCH DENT,DEPT MICROBIOL NAGOYA AICHI 464 JAPAN UNIV CALIF SAN FRANCISCO,DEPT STOMATOL SAN FRANCISCO CA 94143
Titolo Testata:
FEMS microbiology letters
fascicolo: 1, volume: 124, anno: 1994,
pagine: 43 - 48
SICI:
0378-1097(1994)124:1<43:TPMOP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRYPSIN-LIKE PROTEASE; BACTEROIDES-GINGIVALIS; ASACCHAROLYTICUS; HEMAGGLUTININ; DNA;
Keywords:
PORPHYROMONAS GINGIVALIS; TRANSPOSON MUTAGENESIS; BACTERIAL PIGMENTATION; TRYPSIN-LIKE PROTEASE; HEMAGGLUTINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
C.I. Hoover e F. Yoshimura, "TRANSPOSON-INDUCED PIGMENT-DEFICIENT MUTANTS OF PORPHYROMONAS-GINGIVALIS", FEMS microbiology letters, 124(1), 1994, pp. 43-48

Abstract

Trypsin-like protease activity, hemagglutination activity, and accumulation of heme-containing compounds (black pigment) are considered to be virulence factors of Porphyromonas gingivalis. Transposon-mutagenesis was used for the first time to isolate pigment-deficient mutants. These mutants exhibited simultaneous deficiency in trypsin-like protease activity and hemagglutination activity. Two major membrane-associated proteins, observed by SDS-PAGE with the parent strain, were essentially absent from the mutant strains. Immunoblot analysis indicated thatthese two proteins correspond to putative hemagglutinin and hemagglutinin/protease products of P. gingivalis. Each mutant contained only one transposon insertion, thus the pleiotropic phenotype resulted from single site-specific mutations. The results indicate that trypsin-like protease activity is required for accumulation of protoheme from hemoglobin by P. gingivalis and that genetic and/or physiological linkage exists between trypsin-like protease activity and hemagglutination activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 05:58:29