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Titolo:
THE ROLE OF PHOSPHOENOLPYRUVATE IN THE SIMULTANEOUS UPTAKE OF FRUCTOSE AND 2-DEOXYGLUCOSE BY ESCHERICHIA-COLI
Autore:
KORNBERG H; LAMBOURNE LTM;
Indirizzi:
UNIV CAMBRIDGE,DEPT BIOCHEM,TENNIS COURT RD CAMBRIDGE CB2 1QW ENGLAND
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 23, volume: 91, anno: 1994,
pagine: 11080 - 11083
SICI:
0027-8424(1994)91:23<11080:TROPIT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE; PHOSPHOFRUCTOKINASE;
Keywords:
GLUCOSE TRANSPORT SYSTEM; KINETICS OF PHOSPHORYLATIVE TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
H. Kornberg e L.T.M. Lambourne, "THE ROLE OF PHOSPHOENOLPYRUVATE IN THE SIMULTANEOUS UPTAKE OF FRUCTOSE AND 2-DEOXYGLUCOSE BY ESCHERICHIA-COLI", Proceedings of the National Academy of Sciences of the United Statesof America, 91(23), 1994, pp. 11080-11083

Abstract

Nomnetabolizable glucose analogs inhibit the growth of Escherichia coli on a wide variety of carbon sources. This phenomenon was investigated with particular reference to the effect of 2-deoxyglucose (2DG) on growth on fructose as sole carbon source. When the inhibitor is supplied in sufficiently low concentrations, the initial arrest of growth isovercome; this relief of inhibition is aided by means that increase the availability of phosphoenolpyruvate (PEP) to the growing cells, such as the use of L-aspartate instead of ammonium chloride as sole nitrogen source for growth, and the introduction of the pps(+) allele into a pps(-) strain. Studies with [C-14]2DG showed that the analog or its L-phosphate as such did not inhibit growth but that 2DG exerted its effect by competing for intracellular PEP and lowering its concentrationbelow that needed to sustain growth. Direct measurements of the PEP-dependent phosphorylation of 2DG and of fructose by permeabilized E. coli showed that the apparent K-m for PEP was nearly 7 times higher for 2DG than it was for fructose, although the apparent V-max for 2DG was nearly 3 times that for fructose; this explains the ability of cells to overcome the inhibition by low, but not by high, concentrations of 2DG.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 15:42:21