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Titolo:
SPERMINE POTENTIATION OF RECOMBINANT N-METHYL-D-ASPARTATE RECEPTORS IS AFFECTED BY SUBUNIT COMPOSITION
Autore:
ZHANG L; ZHENG X; PAUPARD MC; WANG AP; SANTCHI L; FRIEDMAN LK; ZUKIN RS; BENNETT MVL;
Indirizzi:
ALBERT EINSTEIN COLL MED,DEPT NEUROSCI,1300 MORRIS PK AVE BRONX NY 10461 ALBERT EINSTEIN COLL MED,DEPT NEUROSCI BRONX NY 10461
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 23, volume: 91, anno: 1994,
pagine: 10883 - 10887
SICI:
0027-8424(1994)91:23<10883:SPORNR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; NMDA RECEPTOR; XENOPUS OOCYTES; SINGLE-CHANNEL; REGIONAL DISTRIBUTION; HIPPOCAMPAL-NEURONS; INDUCED CURRENTS; SPLICE VARIANTS; WHOLE-CELL; RAT-BRAIN;
Keywords:
EXCITATORY AMINO ACID RECEPTORS; GLUTAMATE RECEPTORS; RECOMBINANT RECEPTORS; XENOPUS OOCYTE EXPRESSION SYSTEM; POLYAMINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
L. Zhang et al., "SPERMINE POTENTIATION OF RECOMBINANT N-METHYL-D-ASPARTATE RECEPTORS IS AFFECTED BY SUBUNIT COMPOSITION", Proceedings of the National Academy of Sciences of the United Statesof America, 91(23), 1994, pp. 10883-10887

Abstract

The present study shows that both the NR1 and NR2 subunits criticallyaffect spermine potentiation of heteromeric recombinant N-methyl-D-aspartate receptors. NR1(011), the most prominent NR1 splice variant in rat forebrain, and NR1(100) prominent in midbrain, were expressed in Xenopus oocytes singly and in combination with NR2A, NR2B, and NR2C subunits. As for NR1(011) homomers, NR1(011)/NR2B receptors exhibited spermine potentiation by two mechanisms: by increasing glycine affinity and by increasing current through receptors with bound N-methyl-D-aspartate and glycine. NR1(011)/NR2A receptors exhibited only the increase in glycine affinity, and NR1(011)/NR2C receptors exhibited neither. Asfor NR1(100) homomers, NR1(100)/NR2B and NR1(100)/NR2A receptors exhibited spermine potentiation only by increasing the glycine affinity. Spermine produced no potentiation of NR1(100)/NR2C receptors. Thus, theNR2B subunit ''permits'' both forms of spermine potentiation, the NR2A subunit permits spermine potentiation only by increasing the glycineaffinity, and the NR2C subunit permits neither form of potentiation. Spermine actions on NR1/NR2 showed little voltage dependence. These observations are of interest because the NR1 and NR2 subunits are differentially distributed and developmentally regulated. At early postnatalages, NR2B subunit mRNA was more highly expressed than NR2A and NR2C mRNAs in hippocampus, neocortex, and caudate-putamen. These findings account for many of the observed differences among neurons in polyamineactions and suggest that these actions will vary in a cell-specific and age-related manner.

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Documento generato il 11/07/20 alle ore 03:52:20