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Titolo:
MASS-SPECTROMETRY - A TOOL FOR ONLINE MONITORING OF ANIMAL-CELL CULTURES
Autore:
BEHRENDT U; KOCH S; GOOCH DD; STEEGMANS U; COMER MJ;
Indirizzi:
BOEHRINGER MANNHEIM GMBH,NONNENWALD 2 D-82377 PENZBERG GERMANY
Titolo Testata:
Cytotechnology
fascicolo: 3, volume: 14, anno: 1994,
pagine: 157 - 165
SICI:
0920-9069(1994)14:3<157:M-ATFO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; FERMENTATION;
Keywords:
ANIMAL CELLS; LARGE-SCALE CULTIVATION; IN-PROCESS CONTROL; MASS SPECTROMETRY; ONLINE MONITORING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
U. Behrendt et al., "MASS-SPECTROMETRY - A TOOL FOR ONLINE MONITORING OF ANIMAL-CELL CULTURES", Cytotechnology, 14(3), 1994, pp. 157-165

Abstract

The magnetic sector mass spectrometer is able to detect oxygen uptakeand carbon dioxide production rates from animal cell cultivations performed in 101 biorectors. Such data have not been available with the use of classic exhaust gas analysis applying paramagnetic analyzers andinfra-red sensors due to the insensitivity of the apparatus available. In the course of the present work we were able to demonstrate, that the oxygen uptake rate correlates to the number of viable cells. Additionally oxygen uptake rates supplied on-line information about the actual physiology of the cells: When the rates changed during the cultivation process, this immediately indicated the occurrence of limitationsof components in the medium. The information could be useful in timing key events, such as performing splits or harvesting the bioreactor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 17:14:19