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Titolo:
IMPORTANCE OF PRIMER SELECTION IN THE APPLICATION OF PCR TECHNOLOGY TO THE DIAGNOSIS OF BOVINE LEUKEMIA-VIRUS
Autore:
MARSOLAIS G; DUBUC R; BERGERON J; MORREY JD; KELLY EJ; JACKSON MK;
Indirizzi:
MINIST AGR PECHERIES & ALIMENTAT QUEBEC,531 BLVD DES PRAIRIES LAVAL DES RAPIDES H7N 4Z3 PQ CANADA
Titolo Testata:
Journal of veterinary diagnostic investigation
fascicolo: 3, volume: 6, anno: 1994,
pagine: 297 - 301
SICI:
1040-6387(1994)6:3<297:IOPSIT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
G. Marsolais et al., "IMPORTANCE OF PRIMER SELECTION IN THE APPLICATION OF PCR TECHNOLOGY TO THE DIAGNOSIS OF BOVINE LEUKEMIA-VIRUS", Journal of veterinary diagnostic investigation, 6(3), 1994, pp. 297-301

Abstract

The polymerase chain reaction (PCR) was used to detect bovine leukemia virus in bovine blood samples. When applied to leucocytes extracted from the blood samples, the standard method of DNA extraction gave good correlation with agar gel immunodiffusion, but a method in which 5 mul of blood was the starting material was unreliable. Selection of theprimers was important, and differences in results were observed when the PCR method was applied to blood samples from different geographic areas. The sensitivity varied from 50% to 90%, depending on the primerset applied to the gag gene of proviral nucleic acid. This variation was based on geographic origin of the cattle, suggesting an influence of viral strain. In some areas, more than 1 primer may needed to optimize results.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/10/20 alle ore 12:24:50