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Titolo:
THE POTENTIAL PROTECTIVE IMMUNE-RESPONSES TO SYNTHETIC PEPTIDES CONTAINING CONSERVED EPITOPES OF PORPHYROMONAS-GINGIVALIS FIMBRIAL PROTEIN
Autore:
OGAWA T;
Indirizzi:
OSAKA UNIV,FAC DENT,DEPT ORAL MICROBIOL SUITA OSAKA 565 JAPAN
Titolo Testata:
Journal of Medical Microbiology
fascicolo: 5, volume: 41, anno: 1994,
pagine: 349 - 358
SICI:
0022-2615(1994)41:5<349:TPPITS>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; T-CELL; AMINO-ACID; SERUM IGG; PREDICTION; ANTIGENS; ASACCHAROLYTICUS; COLONIZATION; ANTIBODIES; VACCINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
T. Ogawa, "THE POTENTIAL PROTECTIVE IMMUNE-RESPONSES TO SYNTHETIC PEPTIDES CONTAINING CONSERVED EPITOPES OF PORPHYROMONAS-GINGIVALIS FIMBRIAL PROTEIN", Journal of Medical Microbiology, 41(5), 1994, pp. 349-358

Abstract

The immunodominant and T-cell epitopes within the fimbrial subunit protein (fimbrilin) of Porphyromonas ginyivalis strain 381 were analysedby multi-pin peptide synthesis technology. Six regions with immunodominant epitopes within a sequence of 337 amino acids that reacted with the serum of patients with adult periodontitis were detected. T cells from mice immunised with P. gingivalis fimbriae exhibited proliferative responses to P. gingivalis fimbriae or to six 10-mer synthetic peptides from the amino-acid sequence of the fimbrillin. Three synthetic peptides that contained the regions responsible for the immuno-dominant epitopes as well as those which coincided with a T-cell epitope of P. gingivalis fimbrial molecules-FP381(142-161), FP381(202-221) and FP381(216-243)-were selected and synthesised. When guinea-pigs were immunised with fimbriae or one of the three synthetic peptide segments and anadjuvant in Freund's incomplete adjuvant, enhanced production of the antigen-specific IgG antibodies was induced in the serum of the animals. Furthermore, of the three synthetic peptides tested, FP381(202-221)produced the greatest protective immune response in guinea-pigs infected with P. gingivalis and this was more effective than the native fimbrial protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:35:15