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Titolo:
ANALYSIS OF SHORT NOVEL HUMAN PAPILLOMAVIRUS SEQUENCES
Autore:
TACHEZY R; VANRANST MA; CRUZ Y; BURK RD;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PEDIAT,1300 MORRIS PK AVE BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MICROBIOL BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT IMMUNOL BRONX NY 10461 INST HEMATOL & BLOOD TRANSFUS,DEPT EXPTL VIROL CR-10130 PRAGUE CZECH REPUBLIC CATHOLIC UNIV LEUVEN,SCH MED,REGA INST MED RES,DEPT MICROBIOL & IMMUNOL B-3000 LOUVAIN BELGIUM
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 2, volume: 204, anno: 1994,
pagine: 820 - 827
SICI:
0006-291X(1994)204:2<820:AOSNHP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CERVICAL SCRAPES; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
R. Tachezy et al., "ANALYSIS OF SHORT NOVEL HUMAN PAPILLOMAVIRUS SEQUENCES", Biochemical and biophysical research communications, 204(2), 1994, pp. 820-827

Abstract

Consensus primer mediated PCR protocols have the potential to amplifypreviously uncharacterized human papillomavirus (EBV) genotypes. In astudy on 500 cervicovaginal samples, we amplified four sequences (L1AE 1 to L1AE 4) that failed to hybridize to any of the available HPV type-specific oligonucleotide probes. Nucleotide sequencing revealed that the sequences were derived from the L1 region of hitherto unsequenced genotypes. Comparison of phylogenetic trees based on the amplified L1 sequences with E6-derived phylogenetic trees resulted in the identification of L1AE 1 and L1AE 2 as putative novel HPV PCR genotypes. L1AE1 was related to HPV 39, whereas L1AE 2 was related to HPV 51. The L1AE 3 and L1AE 4 sequences occupied L1-phylogenetic branches equivalentto the positions of HPV 66 and HPV 61, respectively, in an E6-phylogenetic tree. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:13:21