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Titolo:
LEUKOCYTE LOW-DENSITY-LIPOPROTEIN RECEPTOR (LDL-R) DOES NOT CONTRIBUTE TO LDL CLEARANCE IN-VIVO - BONE-MARROW TRANSPLANTATION STUDIES IN THE MOUSE
Autore:
FAZIO S; HASTY AH; CARTER KJ; MURRAY AB; PRICE JO; LINTON MF;
Indirizzi:
VANDERBILT UNIV,MED CTR,DEPT MED NASHVILLE TN 37232 VANDERBILT UNIV,MED CTR,DEPT PATHOL NASHVILLE TN 37232 VANDERBILT UNIV,MED CTR,DEPT PHARMACOL NASHVILLE TN 37232 VET ADM MED CTR NASHVILLE TN 37232
Titolo Testata:
Journal of lipid research
fascicolo: 2, volume: 38, anno: 1997,
pagine: 391 - 400
SICI:
0022-2275(1997)38:2<391:LLR(DN>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED HUMAN LYMPHOCYTES; E-DEFICIENT MICE; APOLIPOPROTEIN-E; FAMILIAL HYPERCHOLESTEROLEMIA; CELLS; MACROPHAGES; ATHEROSCLEROSIS; DEGRADATION; BETA; HYPOCHOLESTEROLEMIA;
Keywords:
FAMILIAL HYPERCHOLESTEROLEMIA; MACROPHAGES; BONE MARROW TRANSPLANTATION; GENE DELIVERY; DYSLIPIDEMIAS; MOUSE MODELS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
S. Fazio et al., "LEUKOCYTE LOW-DENSITY-LIPOPROTEIN RECEPTOR (LDL-R) DOES NOT CONTRIBUTE TO LDL CLEARANCE IN-VIVO - BONE-MARROW TRANSPLANTATION STUDIES IN THE MOUSE", Journal of lipid research, 38(2), 1997, pp. 391-400

Abstract

The targeted disruption of the low density lipoprotein (LDL) receptorgene in mice results in accumulation of plasma LDL cholesterol and inpredisposition to diet-induced aortic atherosclerosis. Although the liver is the central organ for receptor mediated clearance of LDL, the in vivo role of other organs and tissues in LDL catabolism has not been directly studied. Since bone marrow-derived cells such as blood leukocytes and tissue macrophages express LDL receptors and contribute a large cell mass to the body, we designed bone marrow transplantation (BMT) experiments to reconstitute LDL receptor null mice [LDL-R(-/-)] with marrow obtained from LDL-R wild-type mice [LDL-R(+/+)] and evaluatethe effects on parameters of plasma lipid metabolism. Although reconstitution of the transplanted mice with donor bone marrow cells was complete, no differences in plasma lipid levels and lipoprotein distribution were found between groups, irrespective of the diet used, and turnover studies using I-125-labeled LDL showed that LDL receptor expression by leukocytes and macrophages does not significantly contribute to plasma LDL clearance. The complementary experiment of transplanting LDL-R(-/-) marrow into C57BL/6 recipients [LDL-R(-/-)-->LDL(+/+)], performed to evaluate the role of leukocyte LDL-R in normocholesterolemic conditions, also produced no effects on plasma lipid parameters. LDL binding studies using macrophages isolated from transplanted mice showeda lack of LDLR expression. Thus, despite their large number and wide distribution, bone marrow-derived cells do not significantly influencereceptor-mediated clearance of plasma LDL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:59:03