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Titolo:
CRUCIAL ROLE OF INTERFERON CONSENSUS SEQUENCE BINDING-PROTEIN, BUT NEITHER OF INTERFERON REGULATORY FACTOR-1 NOR OF NITRIC-OXIDE SYNTHESIS FOR PROTECTION AGAINST MURINE LISTERIOSIS
Autore:
FEHR T; SCHOEDON G; ODERMATT B; HOLTSCHKE T; SCHNEEMANN M; BACHMANN MF; MAK TW; HORAK I; ZINKERNAGEL RM;
Indirizzi:
UNIV ZURICH HOSP,INST EXPT IMMUNOL,SCHMELZBERGSTR 12 CH-8091 ZURICH SWITZERLAND UNIV ZURICH HOSP,DEPT INTERNAL MED CH-8091 ZURICH SWITZERLAND UNIV ZURICH HOSP,DEPT PATHOL CH-8091 ZURICH SWITZERLAND RES INST MOL PHARMACOL,DEPT MOL GENET D-12207 BERLIN GERMANY PRINCESS MARGARET HOSP,ONTARIO CANC INST TORONTO ON M4X 1K9 CANADA
Titolo Testata:
The Journal of experimental medicine
fascicolo: 5, volume: 185, anno: 1997,
pagine: 921 - 931
SICI:
0022-1007(1997)185:5<921:CROICS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; TRANSCRIPTION FACTOR IRF-1; BLOOD-DERIVED MACROPHAGES; CELL-INDEPENDENT PATHWAY; GAMMA-INTERFERON; MICE LACKING; INTRACELLULAR BACTERIUM; LISTERICIDAL ACTIVITY; TARGETED DISRUPTION; ANTIVIRAL DEFENSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
T. Fehr et al., "CRUCIAL ROLE OF INTERFERON CONSENSUS SEQUENCE BINDING-PROTEIN, BUT NEITHER OF INTERFERON REGULATORY FACTOR-1 NOR OF NITRIC-OXIDE SYNTHESIS FOR PROTECTION AGAINST MURINE LISTERIOSIS", The Journal of experimental medicine, 185(5), 1997, pp. 921-931

Abstract

Listeria monocytogenes is widely used as a model to study immune responses against intracellular bacteria. It has been shown that neutrophils and macrophages play an important role to restrict bacterial replication in the early phase of primary infection in mice, and that the cytokines interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) are essential for protection. However, the involved signaling pathways and effector mechanisms are still poorly understood. This study investigated mouse strains deficient for the IFN-dependent transcription factors interferon consensus sequence binding protein (ICSBP), interferon regulatory factor (IRF) 1 or 2 for their capacity to eliminate Listeria in vivo and in vitro and for production of inducible reactive nitrogen intermediates (RNI) or reactive oxygen intermediates (ROI) in macrophages. ICSBP-/- and to a lesser degree also IRF2(-/-) mice were highly susceptible to Listeria infection. This correlated withimpaired elimination of Listeria from infected peritoneal macrophage (PEM) cultures stimulated with IFN-gamma in vitro, in addition these cultures showed reduced and delayed oxidative burst upon IFN-gamma stimulation, whereas nitric oxide production was normal. Tn contrast, micedeficient for IRF1 were not able to produce nitric oxide, but they efficiently controlled Listeria in vivo and in vitro. These results indicate that (a) the ICSBP/IRF2 complex is essential for IFN-gamma-mediated protection against Listeria and that (b) ROI together with additional still unknown effector mechanisms may be responsible for the anti-lister ia activity of macrophages, whereas IRF1-induced RNI are not limiting.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:46:06